TFPI-2和MMP-9在非小细胞肺癌中的表达及临床意义

Expression and clinical significance of TFPI-2 and MMP-9 in non-small cell lung cancer

目的:研究组织因子途径抑制物-2(tissue factor pathway inhibitor-2,TFPI-2)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及之间的相关性。方法:采用免疫组化法检测60例NSCLC组织TFPI-2和MMP-9的表达,并分析TFPI-2和MMP-9的表达水平与性别、年龄、组织类型、分化程度、肿瘤直径、TNM分期及淋巴结转移的关系。结果:NSCLC中临床分期为Ⅰ、Ⅱ、Ⅲ期的患者中TFPI-2表达阳性率分别为75.8%、25.0%和40.0%(P<0.01),无淋巴结转移和有淋巴结转移的患者中TFPI-2表达阳性率分别为66.7%、38.1%(P<0.01),肿瘤直径>5cm和≤5cm的患者TFPI-2表达阳性率分别为15.0%、77.5%(P<0.01)。临床分期为Ⅰ、Ⅱ、Ⅲ期的患者中MMP-9表达阳性率分别为48.5%、83.3%和93.3%(P<0.01),无淋巴结转移和有淋巴结转移的患者中MMP-9表达阳性率分别为56.4%、85.7%(P<0.01),肿瘤直径>5cm和≤5cm的患者MMP-9表达阳性率分别为95.0%、52.5%(P<0.01)。NSCLC组织中TFPI-2的低表达与MMP-9的高表达呈负相关(r=-0.476,P=0.001)。结论:TFPI-2的低表达和MMP-9的高表达与NSCLC临床分期、肿瘤大小及淋巴结转移密切相关。TFPI-2可能通过抑制MMP-9的表达,从而抑制NSCLC的生长、浸润和转移。

Objective：To investgate the relationship between the expression and correlation of TFPI-2 and MMP -9 in NSCLC.Methods：The expression of TFPI-2,MMP-9 was detected by immunohistochemistry in 60 patients with NSCLC.Results：In patients with NSCLC of clinical stage Ⅰ,Ⅱ,Ⅲ TFPI-2 expression positive rates were 75.8％,25.0％ and 40.0％ respectively（P ＜ 0.01）.TFPI-2 expression positive rates without lymph node metastasis and lymph node metastasis in patients were 66.7％ and 38.1％ （P＜0.01）.In tumor diameter ＞ 5 cm and ≤ 5cm patients TFPI-2 expression positive rates were 15.0％,77.5％ （P ＜ 0.01）.In clinical stage Ⅰ,Ⅱ,Ⅲ patients MMP-9 expression positive rates were 48.5％,83.3％,93.3％ （P ＜ 0.01） respectively.In patients without lymph node metastasis and lymph node metastasis MMP-9 expression positive rates were 56.4％,85.7％ （P ＜ 0.01）.Tumor diameter ＞ 5 cm and ≤ 5 cm patients MMP-9 expression positive rates were 95.0％,52.5％ （P ＜ 0.01）.The expression of TFPI-2 and MMP-9 in NSCLC were negatively correlated （r =-0.476,P =0.001）.Conclusion：The low expression of TFPI-2 and high expression of MMP-9 are closely related with NSCLC clinical stage,tumor size and lymph node metastasis.TFPI-2 could inhibit the expression of MMP-9 to prevent tumor growth,invasion and metastasis.