贵州省彝族、瑶族及汉族乙肝病毒易感性与白介素-10基因的相关性

The Association of HBV Susceptibility with Interleukin-10 Gene Polymorphism in Yi,Yao,and Han Ethnics of Guizhou Province

目的:研究贵州省彝族、瑶族以及汉族人群白介素-10(IL-10)基因多态性与乙型肝炎病毒易感性的相关性。方法:对研究人群中肝病毒感染者和非感染者采用聚合酶链式反应-限制性片段长度多态性(PCRRFLP)方法分析IL-10基因启动子区-592位点多态性,序列特异性引物-聚合酶链式反应(PCR-SSP)方法分析IL-10基因启动子区-819位点多态性,短串联重复序列或微卫星DNA-聚合酶链式反应(STRs-PCR)方法分析IL-10基因启动子区IL-10.G和IL-10.R两个STR位点。结果:IL-10基因位点单倍型与HBV感染的相关分析表明,贵州省彝族乙肝病毒感染组(HBsAg、抗-HBs或抗-HBc中任一项阳性者)IL-10.G.461bp(20CA)-592A-819T单倍型频率显著高于乙肝病毒非感染组(P<0.05),非感染组IL-10.G.459bp(19CA)-592 C-819 C单倍型频率显著高于感染组(P<0.05);贵州省汉族乙肝病毒非感染组IL-10.G.459bp(19CA)-592 A-819 T单倍型频率显著高于感染组(P<0.05),贵州省瑶族乙肝病毒感染组与非感染组之间各单倍型频率分布差异均无统计学意义(P>0.05)。结论:IL-10-592、IL-10-819以及IL-10.G、IL-10.R位点多态性在贵州世居彝族、瑶族以及汉族中有不同的分布,并且可能与该人群对乙肝病毒的易感性相关。

Objective： To investigate the association of IL-10 gene polymorphism withthe susceptibility to hepatitis B virus infection in Han, Yi, and Yao ethnics of Guizhou. Methods： Five hundred volunteers from Guizhou province were chosen for the investigation. Allelic frequencies of IL-10-592 were identified by PCR-RFLP. Allelic frequencies of IL-10-819 were identified by PCR-SSP. Allelic frequencies of IL-10. G and IL-10. R loci were identified by STRs-PCR. Results：The haplotype of IL-10. G. 461bp （20CA）-592A-819T accompanied with higher HBV infection rate in Yi ethnic. The haplotype of IL-10. G 459bp （19CA）-592C-819C seemed to associated with lower HBV infection rate in Yi ethnic. The haplotype of IL-10. G 459bp （19CA）-592A-819T showed lower HBV infection rate in Han ethnic. Haplotype frequencies of these genes did not show differences between infected group and noninfected group in Yao ethnic （P 〉0.05）. Conclusion： IL-10-592, IL-10-819 and IL-10. G polymorphisms influence susceptibility to HBV infection in Han, Yi, and Yao ethnics of Guizhou.