液相色谱-四极杆-线性离子阱质谱仪联用法对护肤类化妆品中7种性激素的快速筛查

Rapid Screening and Confirmation of 7 Sex Hormones in Cosmetics by High Performance Liquid Chromatography-Q-Trap Mass Spectrometry

目的：建立液相色谱-四极杆-线性离子阱质谱（LC-Q-Trap）法测定护肤类化妆品中7种性激素含量的方法.方法：样品用含1.0％乙酸的乙腈溶液超声溶解,用C18柱（2.1 mm×100 mm,1.7μm）分离,乙腈和0.1％乙酸为流动相梯度洗脱,用正、负离子模式同时扫描检测样品中7种性激素含量.结果：7种性激素在0.060 06 ～1.008 9 mg/L范围内呈良好的线性相关性,相关系数均大于0.9991,定量限为2.0～5.0 μg/L,平均回收率为88.7％～110.7％,相对标准偏差为2.7％ ～6.2％.结论：LC-Q-Trap联合检测方法适用于化妆品中性激素的快速筛查和测定.

Objective ： To establish a method for determination of sex hormone in cosmetics with high performance liquid chromatography-Q-Trap （LC-Q-Trap） mass spectrometry. Method：Samples were ultrasonic dissolved in 1.0% acetic acid （in acetonitrile） and separated on C18 column （2. lmm × 100mm, 1.71μm） , and eluted by using acetonitrile, and 0.1% acetic acid as mobile phase; The mass analyzer was performed in positive and negative full scan modes in one injection at the same time. Results：The linear ranges of the 7 sex hormones were 0. 060 06 ～1. 008 9 mg/L with con＇elation coefficients above 0. 999 1. The limits of quantification were 2.0 ～ 5.0 p,g/L, and the recoveries were 88. 7 ～110.7% % with relative standard deviations of 2.7 ～ 6.2%. Conclusion： LC-Q-Trap combined method is suitable for rapid screening and determination of sex hormones in cosmetics