胃癌组织中TRβ1和β-catenin的表达及其临床意义

Expressions and clinical significance of TRβ1 and β-catenin in gastric cancer

目的:探讨甲状腺激素受体β1(thyroid hormone receptorβ1,TRβ1)和β连环蛋白(β-catenin)在胃癌组织中的表达及其临床意义。方法:应用免疫组化SP法和RT-PCR技术检测80例胃癌组织及40例无癌胃黏膜组织中TRβ1和β-catenin蛋白、mRNA的表达,分析两者mRNA表达量与临床病理指标的关系。结果:胃癌组织和无癌胃黏膜组织中TRβ1蛋白的阳性表达率分别为16.25%(13/80)、80.00%(32/40);β-catenin蛋白的异常表达率分别为62.50%(50/80)、0(0/40)。TRβ1 mRNA在无癌黏膜组织中的表达量高于癌组织(P<0.01),β-catenin mRNA则相反(P<0.01),且两者的表达量与肿瘤分化、淋巴结转移、浸润深度、TNM分期有关(P<0.05)。结论:胃癌组织中TRβ1蛋白和mRNA的表达均减少,β-catenin则增加;两者mRNA表达量与胃癌的生物学行为有关。TRβ1和β-catenin有可能参与胃癌的发生、发展和侵袭转移。

Objective：To explore the expression and clinical significance of TR β 1 and β-catenin in gastric cancer.Methods：Immunohistochemical SP method was used to detect the expression of TR β 1 and β-catenin protein,and RT-PCR was used to test their mRNA in 80 human gastric carcinomas and 40 noncancerous gastric tissues.Then,the correlation between the mRNA expression quantity and clinical indicators was analysed.Results：The positive expression rate of TRβ1 protein in gastric carcinomas and noncancerous gastric tissues was 16.25％ （13/80） and 80.00％ （32/40）,respectively.The abnormal expression rate of β-catenin protein was 62.50％ （50/80） and 0（0/40）,respectively.TR β 1 mRNA expression quantity was higher in noncancerous gastric tissues than that in gastric carcinomas,whereas β-catenin mRNA presented an opposite tendency.（P＜0.01）.Both TRβ1 mRNA and β-catenin mRNA expression quantity were related with tumorous differentiation,lymphatic metastasis,infiltration and TNM stage.Conclusion：TRβ1 expression quantity is decreased,whether at the level of protein or mRNA,but β-catenin is increased at the both level.TR β 1 and β-catenin＇s mRNA quantity relate to tumorous biological behaviour.Therefor,TRβ1 and β-catenin probablely involve in gastric carcinoma＇s occurrence,development,aggression and metastasis.