云南腾冲雪鸡肌肉蛋白质组学研究

Proteomic Analysis of Tengchong Snowcock Muscles in Yunnan

腾冲雪鸡是云南省腾冲县的一个地方优良家鸡品种,具有羽毛洁白、肉骨乌黑、肉质好、抗病性强等优良特性。本研究以140日龄腾冲雪鸡为对象,采用双向电泳(DE)分析其腿肌与胸肌中蛋白表达差异,并以基质辅助激光解析电离-串联飞行时间质谱(MALDI-TOF/TOF MS)鉴定腿肌与胸肌中差异蛋白质点。结果表明,经双向电泳图谱分析所获得的肌肉组织蛋白点分布于pH4.0～7.0之间,蛋白点清晰,图谱分辨率较好,其中腿肌中检测到1421±34个蛋白点,胸肌中检测到1329±25个蛋白点,对比分析腿肌和胸肌组织的DE图谱,发现有110个点存在明显的表达差异,在腿肌中高表达的点有56个,在胸肌中高表达的点有54个;选取的蛋白质点经鉴定为核纤层蛋白A、磷酸葡萄糖变位酶1、β-烯醇酶、超氧化物歧化酶、肌酸激酶M型、乙二醛酶1、原肌原蛋白1,以及一些假设蛋白和非特征性蛋白。

Tengchong snowcock,with some unique genetic characteristics,such as white feather,black meat and bone,good meat quality and high disease resistance,is an indigenous chicken breed discovered in recent years in Tengchong county of Yunnan Province.In the present study,140-day-old Tengchong snowcocks were chosen for comparative analysis of protein expression in thigh and breast muscles by two-dimensional gel electrophoresis（2-DE） as well as identification of the proteins with different expression levels in both muscles by peptide mass fingerprinting with matrix-assisted laser desorption ionization-time of flight-time of flight-mass spectrometry（MALDI-TOF/TOF MS）.The 2-DE analysis displayed high-resolution clear protein spots distributed in the the range of pH 4.0 to 7.0,and 1421±34 and 1329 ± 25 spots were observed for thigh and breast muscles,respectively.The results of comparative analysis illustrated a significant difference in the expression levels of 110 spots in the 2-DE images of both muscles,of which 56 were highly expressed in thigh muscle while 54 in breast muscle.The protein spots were identified as lamin-A,phosphoglucomutase 1,beta-enolase,superoxide dismutase,creatine kinase M-type,glyoxylase 1,isoform 1 of tropomyosin alpha-1 chain,and some hypothetical proteins and uncharacterized proteins,respectively.