珍黄安宫片质量标准研究

Study on Quality Standard for Zhenhuang Angong Tablets

目的：建立珍黄安宫片质量标准。方法：采用TLC法鉴别青黛、黄芩提取物、石菖蒲、小檗根提取物,采用HPLC法测定珍黄安宫片中大黄的活性成分大黄素、大黄酚、大黄素甲醚的含量。结果：TLC色谱申可检出青黛、黄芩提取物、石菖蒲、小檗根提取物,阴性均无干扰。大黄素在0.033~0.669μg范围内,线性关系良好,平均回收率100.77%（RSD=1.40%,n=6）;大黄酚在0.102~2.034μg范围内,线性关系良好,平均回收率100.02%（RSD=1.00%,n=6）;大黄素甲醚在0.033~0.659μg范围內,线性关系良好,平均回收率100.60%（RSD=1.03%,n=6）。结论：该方法准确,重复性好。可用于珍黄安宫片的质量控制。

Objective： To establish a method for the quality standard of Zhenhuang Angong tablets. Method： Indigo Naturalis, Scutellariae Paeonia Radix Extract, Acori Tatarinowii Resina and Berberidis Radix Extract were identified by TLC. The content of emod- in, chrysophano and physcion were determined by HPLC. Result： Indigo Naturalis, Scutellariae Paeonia Radix Extract, Acori Tatarinowii Resina and Berberidis Radix Extract could be identified by TLC, and the blank test showed the other medicines had no interfer- ence. The methodological study showed that a good linear correlation was within the range of 0.033-0.669 μg for emodin, 0. 102- 2. 034 μg for chrysophanol and 0.033-0. 659 μg for physcion. The average recovery of emodin was 100.77% （ RSD = 1.40% ,n = 6）. The average recovery of chrysophanol was 100.02% （ RSD = 1.00% , n =6）. The average recovery of physcion was 100.60% （ RSD = 1.03% , n = 6）. Conclusion： The method is accurate with good reproducibility, and suitable for the quality control of Zhenhuang An- gong tablets.