HPLC法测定银黄颗粒中黄芩苷、黄芩素、绿原酸和咖啡酸的含量

Determination of Baicalin,Baicalein,Chlorogenic Acid and Caffeic Acid in Yinhuang Granules by HPLC

目的:建立HPLC法测定银黄颗粒中黄芩苷、黄芩素、绿原酸和咖啡酸的含量。方法:色谱柱为ZOBRAX Extend-C_(18)(250mm×4.6mm,5μm),流动相为乙腈-1%冰醋酸溶液,梯度洗脱;流速1.0ml·min^(-1),柱温30℃,检测波长327nm。结果:4个成分在各自浓度范围内线性关系良好(r≥0.9995),加样回收率为97.3%~99.1%。结论:该法操作简便快捷,准确可靠,重复性好,可用于测定银黄颗粒中黄芩苷、黄芩素、绿原酸和咖啡酸的含量。

Objective： To establish a determination method for baicalin, baicalein, chlorogenic acid and caffeic acid in Yinhuang granules by HPLC. Method： A ZOBRAX Extend-C18 column（250 mm ×4.6 mm,5 μm） was used, and acetonitrile-1% glacial acetic acid was used as the mobile phase with gradient elution at the flow rate of 1.0 ml·min^- 1. The column temperature was 30℃ and the detection wavelength was at 327nm. Result： The four compounds all showed good linearity within the range of the tested concentra- tions. The average recoveries were within the range of 97.3%-99.1%. Conclusion： The method is simple, accurate and reproduci- ble, and can be used in the determination of baicalin, baicalein, chlorogenic acid and caffeic acid in Yinhuang Granules.