摘要
目的:建立同时测定人参三七颗粒中人参皂苷Rg_1、Re、Rb_1和三七皂苷R_1含量的方法。方法:采用HPLC法,色谱柱为Sunfire C_(18)(150mm×4.6mm,5μm)分析柱.流动相以乙腈-水梯度洗脱;检测波长为203nm;柱温30℃;流速1.0ml·min^(-1)。结果:人参皂苷Rg_1,Re,Rb_1和三七皂苷R_1之间有较好的分离度,4种成分在线性范围內与峰面积之间线性关系良好,人参皂苷Rg_1、Re、Rb_1和三七皂苷R_1加样回收率分别为99.83%,97.84%,98.43%,97.34%,RSD分别为2.08%,1.66%,1.73%和1.42%(n:5)。结论:本方法可同时测定人参三七颗粒中的人参皂苷Rg_1,Re,Rb_1和三七皂苷R_1含量。
Objective： To establish a method for simultaneous determination of ginsenoside Rgl, ginsenoside Re^ginsenoside Rbl and notoginsenoside R1 in Renshen Sanqi granules. Method： The four components in Renshen Sanqi granules were determined simul- taneously by HPLC. A Sunfire CIS （150 mm ×4.6 mm,5 μm） analytical column was used with acetonitrile-water as the mobile phase with gradient elution. The detection wavelength was at 203nm, the flow rate was 1.0 ml · min ^-1 and the column temperature was at 30℃. Result： The relationship between the concentration and the peak area of ginsenoside Rg1,ginsenoside Re,ginsenoside RbI and notoginsenoside R1 was respectively linear. The average recovery was 99.83% ,97.84% ,98.43% and 97.34% with RSD of 2.08% , 1.66% ,1.73% and 1.42% （n = 5 ）, respectively. Conclusion： The method can simultaneously determine the four components in Renshen Sanqi granules.
出处
《中国药师》
CAS
2013年第10期1527-1528,共2页
China Pharmacist
