独花兰组织培养研究

Study on Tissue Culture of Changnienia amoena

[目的]研究独兰花的组织培养方法.[方法]以独兰花假鳞茎为外植体,以1/2MS ＋0.5 mg/L 6-BA ＋0.05 mg/L NAA为芽诱导培养基,以1/2MS＋ 1.5 mg/L 6-BA＋ 0.05 mg/L NAA为生根培养基,研究外植体的芽诱导和生根情况.[结果]外植体消毒的最佳方式为75％酒精30 s＋0.2％ HgCl 6～7 min；试验外植体的污染率、诱导率、死亡率、成活率、生根率分别为14.3％、57.1％、28.6％、50.0％、33.9％.[结论]该研究为独花兰的规模化生产提供了技术指导.

[ Objective ] The aim was to study the tissue culture method of Changnienia amoena. [ Method ] Using the pseudobulb of C. amoe- na as explant, the 1/2MS ＋0.5 mg/L 6-BA ＋ 0.05 mg/L NAA as bud induction medium and the 1/2MS ＋ 1.5 mg/L 6-BA ＋ 0.05 mg/L NAA as rooting medium, the conditions of bud induction, and rooting of the explants were studied. [ Result] The best sterilized method of the explants was 75% alcohol for 30 s and O. 2% HgC1 6 - 7 min ; the contamination rate, induction rate, mortality rate, survival rate and rooting rate of the explants were 14.3% , 57.1% , 28.6%, 50% , 33.9% respectively. [ Conclusion] The study provided technical guidance for the large - scale production of C. amoena.