苦豆子丛枝病植原体的分子检测与鉴定

Molecular Detection and Identification of Sophora alopecuroides Witches'-broom Phytoplasma

【目的】对苦豆子丛枝病植原体做出分子检测与鉴定。【方法】通过PCR扩增技术,分别利用植原体16S rRNA基因,16S-23S rRNA间区及tuf基因序列的通用引物对表现丛枝病症状的苦豆子总DNA进行扩增,得到约1.2、0.3和0.8 kb特异片段。将所得片段分别进行克隆、测序及序列同源性分析。【结果】苦豆子丛枝病植原体(SAWB)上述3序列与榆树黄化组B亚组(16SrV-B)的枣疯病植原体(JWB)相应序列的同源性最高。【结论】苦豆子丛枝病植原体为16Sr V-B亚组成员。

[ Objective] To identify the sophora alopeeuroides witches＇-broom phytoplasma. [ Method] Using PCR with universal primers for phytoplasmal 16S rRNA gene, 16S- 23S rRNA space region and tuf genes, 1.2 kb, 0.3 kb and 0.8 kb DNA fragments were amplified from the total DNA of sophora alopecu- roides that showed witches＇-broom symptom. Amplieons were cloned and sequenced and homology was ana- lyzed. [ Result] The results showed that the 16S rRNA gene, 16S -23S rtlNA space region and tuf gene of phytoplasma isolated from Sophora alopecuroides witches＇-broom phytoplasma （SAWB） had the highest simi- larity with that of Jujube witches＇- broom phytoplasma （JWB） which belonged subgroup B of ＂Elm yellows group＇（ 16SrV- B）. [ Conclusion] Sophora alopecuroides witches＇-broom phytoplasma was identified as the member of 16Sr V - B subgroup.