ILF3蛋白对雌激素受体beta的调控研究

Research on Regulation of Estrogen Receptor Beta by ILF3 Protein

雌激素在体内的生理作用通过雌激素受体(Estrogen Receptor)ERβ和ERα的介导来实现调控。ERβ在乳腺癌、前列腺癌、结肠癌和卵巢癌的发生、发展过程中具有调控作用。ILF3是RNA结合蛋白中的一员,参与细胞周期调控。凝胶阻滞实验(EMSA)证明了ILF3蛋白能结合ERβmRNA 3'UTR。雌激素受体可介导雌激素实现其功能,ILF3蛋白可能通过与ERβ结合参与雌激素信号通路的调控。为探讨他们的关系以及调控机制,构建ILF3过表达的慢病毒载体,通过Western blot检测ERβ蛋白的表达情况。在蛋白水平上,ERβ蛋白的表达量随着ILF3蛋白量的升高而升高,ILF3对ERβ起正向调控。MTT实验检测ILF3基因表达对MCF-7细胞活力有影响。

The physiological effect of estrogen in vivo is regulated through the mediation of estrogen receptors ERβ and ERα.ERβ has regulating effect in the occurrence and development process of breast cancer,prostatic cancer,colon cancer and ovarian cancer.ILF3 is a member of RNA binding protein and participates in cell cycle regulation.Electrophoretic mobility shift assay （EMSA） proves that ILF3 protein can bond with ERβ mRNA 3＇UTR.Estrogen receptor can mediate estrogen receptor to realize its function and ILF3 protein might participate in the regulation of signal channel of estrogen receptor through binding with ERβ.To discuss their relationship and regulatory mechanism,this paper establishes lentiviral vector of ILF3 overexpression and detects the expression of ERββ protein through western blot.In terms of protein level,the expression quantity of ERβ protein increases with the increase of ILF3 protein quantity and ILF3 has positive regulation effect on ERβ.MTT experiment detects that ILF3 gene expression has influence on MCF-7 cell viability.