铁皮石斛内生细菌的分离鉴定与酶活性分析

Separation and Identification of Endophytic Bacteria in Dendrobium Officinale and Analysis on Enzyme Activity

从铁皮石斛组织中分离获得内生细菌52株,其中根部分离得到42株,茎部分离得到10株。编号为FHGXJ7、FHGXJ15-1、FHJXJ3、FHJXJ5的4株菌株在NA培养基上生长较快、菌落较大且菌落数较多,为优势种群。对优势菌株进行鉴定及酶活性检测,结果表明,4株菌株分别鉴定为芽孢杆菌属未知种、苏云金芽孢杆菌、木糖氧化无色杆菌和解淀粉芽孢杆菌,这些菌具有不同程度的蛋白酶、淀粉酶、卵磷脂酶和固氮酶活性。

52 strains of endophytic bacteria are obtained by separation from dendrobium officinale tissue,among which 42 are separated from the root and 10 are separated from the stem.Four strains respectively numbered as FHGXJ7,FHGXJ15-1,FHJXJ3 and FHJXJ5 have rapid growth and large and many bacterial colonies in NA culture medium and are dominant population.The result of identification of dominant strains and enzyme activity test shows that four strains are respectively an unknown species of genus bacillus,bacillus thuringiensis,achromobacter xylosoxidans and bacillus amyloliquefaciens,which have different degrees of activity of protease,amylase,lecithinase and nitrogenase activity.