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复制缺陷型腺病毒PCA的构建及其对结肠癌细胞生长的影响 被引量：1

Establishment of Replication-deficient Recombinant Adenovirus PCA and Its Influence on Cell Growth of Colon Cancer
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摘要 通过同源重组方式,利用Adeasy系统成功构建携带p14ARF基因的复制缺陷型腺病毒,命名为AdpShuttle-CMV-p14ARF(PCA),并研究其对结肠癌细胞株SW480及HT-29的杀伤作用。PCR方法鉴定阳性重组子病毒;MTT法检测PCA对细胞增殖的影响;Hoechst33258荧光染色法检测细胞凋亡形态学变化;结晶紫染色法定性分析PCA对两种结肠癌细胞的杀伤作用。结果显示:经目的病毒PCA作用,SW480及HT-29出现明显病变和生长抑制现象,并呈现出时间和浓度依赖性;Hoechst染色后观察到细胞内出现凋亡小体,并且数目随药物处理浓度的升高而增多。 This paper successfully establishes replication-deficient recombinant adenovirus called AdpShuttle-CMV-p14ARF （PCA） which carries p14ARF gene using Adeasy system through homologous recombination; studies its lethal effect on colon cancer cell strains SW480 and HT-29; identifies positive recombinant subvirus with PCR method; detects the influence of PCA on cell proliferation with MTT method; detects morphological change of cell apoptosis with Hoechst33258 fluorescent staining method; and conducts qualitative analysis on the lethal effect of PCA on two colon cancer cells with crystal violet staining.The result shows that SW480 and HT-29 have obvious lesion and growth inhibition phenomena and present time and concentration dependence under the action of target virus PCA; apoptotic body occurs in cells after Hoechst staining and its number increases with the increase of medication concentration.
作者 黄越 陈文铎 周炜 董淼 高永 许韶威 徐涛 唐斌
机构地区 浙江理工大学生命科学学院
出处 《浙江理工大学学报（自然科学版）》 2013年第6期901-905,918,共6页 Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金 国家自然基金资助项目(30721712)
关键词 Ad-p14-ARF 结肠癌 MTT 结晶紫染色 细胞凋亡 Ad-p14-ARF colon cancer MTT crystal violet staining apoptosis
分类号 Q789 [生物学—分子生物学]
  • 相关文献

参考文献12

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二级参考文献15

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共引文献4

同被引文献18

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浙江理工大学学报（自然科学版）
2013年 第6期

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