摘要
一般甜菊叶中甜菊糖苷的HPLC检测样品处理复杂，采用简易超声波水提取法处理样品，建立快捷分析方法。甜菊糖苷在300一l500I,zg／mL内呈线性关系，相关系数为0．9998，平均加标回收率98．32％，相对标准偏差O．63％。该方法操作简单、重复性好、稳定性好、灵敏度高，可为甜菊叶中甜菊糖苷的测定提供参考。
This article establishes a method of simple ultrasonic water extraction for determination of stevioside, providing a fast and reliable process for analysis of Stevia leaf, The hnear ranger for stevioside is 300-1 500 μg/mL with a regression coefficient of 0.999 8. The average recoveries of stevioside are 98.32%, RSD=0.63%. This method is simple operation, good reproducibility, high sensitivity, stable and reliable determination for stevioside in Stevia rebaudiana Bertohi.
出处
《农产品加工（下）》
2013年第10期14-15,18,共3页
Farm Products Processing
关键词
高效液相色谱(HPLC)
甜菊糖苷
甜菊叶
High Performance Liquid Chromatography （HPLC）
stevioside
Stevia rebaudiana Bertohi
