军牧1号白猪、杜洛克猪、西藏小型猪和大白猪ESR、FSHβ、EGF基因的多态性分布

The polymorphism distribution of gene ESR,FSHβ,and EGF in Junmu 1 white pigs,Durocs,Tibet mini-pigs and Large White

ESR、FSHβ和EGF基因与猪繁殖性状密切相关。为检测军牧1号白猪、西藏小型猪、杜洛克猪和大白猪ESR、FSHβ和EGF基因的多态性分布情况,采用PCR和PCR-RFLP的方法对61头军牧1号白猪,51头杜洛克猪,51头西藏小型猪和69头大白猪的ESR、FSHβ和EGF基因多态性进行了检测。结果显示,对于各个基因的优势等位基因,在军牧1号白猪群体中,ESR基因位点A等位基因频率为0.639 3,FSHβ基因的B等位基因频率高达0.909 8,EGF基因的A等位基因频率仅有0.016 4;杜洛克猪群体中,ESR基因A等位基因频率为0.607 8,FSHβ基因的B等位基因频率为0.823 5,而EGF基因的A等位基因频率仅为0.029 7;西藏小型猪群体中,ESR基因A等位基因频率是0.460 8,FSHβ基因B等位基因频率仅为0.068 7,EGF基因A等位基因频率为0.196 1;大白猪群体中,ESR基因位点有频率为0.500 0的有害A等位基因,而FSHβ基因和EGF基因的B等位基因频率则分别为0.801 3和0.739 1。所有基因型分布均符合哈代-温伯格平衡(P>0.05)。

ESR,FSHβand EG-F genes are correlated with reproduction traits in swine. In order to analyze the polymorphism distribution of ESR,FSHi3,and EGF genes,this study detected the gen- otype of these genes in 61 Junmu 1 white pigs,51 Durocs,51 Tibet mini pigs and 69 Large White with PCR and PCR-RFI.P. The result showed that for the advantageous allele,in Junrnu 1 white pigs,the allelic frequency of A of ESR gene is 0. 639 3,tA of FSHβ 0. 909 8,A of EGF 0. 016 4. In Durocs,A of ESR is 0. 607 8 ,B of FSHβ 0. 823 5,and A of EGF 0. 029 7. For Tibet mini-pigs,A of ESR is 0. 460 8,and B of FSHβ 0. 909 8,A of EGF 0. 196 1. In Large White,A of ESR is 0. 500 0,B of FSHβ and EGF 0. 801 3 and 0.7,＇39 1 ,respectively. The distribution of all genes fit in with Har- dy Weinberg equilibrium （P〈0.05）.