理化诱变亚麻M_1农艺性状的初步分析

Preliminary Analysis of Agronomic Traits on M_1 Flax Induced by Physical and Chemical Agents

为了给亚麻种质创新和品种遗传改良提供优质种质资源,利用60 Co-γ射线(200Gy、350Gy和500Gy)和甲基磺酸乙酯(EMS)(0.2%处理30h和0.3%处理24h)分别对亚麻黑亚19种子进行诱变处理,处理后在田间进行M1农艺性状的观察和分析。结果表明:经过理化诱变的亚麻在M1即可得到叶色、叶形、株高、分枝、茎粗、花色、花形和不育等多种性状变异的突变体,为亚麻突变体库的构建奠定了良好的基础。研究还发现,理化诱变对亚麻M1成苗率和种子产量的影响有所不同。高剂量60 Co-γ射线诱变的亚麻成苗率较低,但种子产量不低;高浓度EMS诱变的亚麻成苗率较高,但大多数是不育植株,导致种子产量较低。

In order to provide high quality germplasm resources for germplasm innovation and the improvement of variety genetic for flax,through 60 Co-7 ray（200 Gy,350 Gy and 500 Gy）and Ethyl Methan Sulfonate（0.2% EMS for 30 h and 0.3% EMS for 24 h） ,the seeds of flax（Linurn usitatissirnurn L. ）from variety Heiya19 were treated,and the agronomic traits in M1 were observed and analyzed in the field. The results showed that the mutants with mutative traits including the mutation of leaf color,leaf shape, plant height, number of branches, diameter of main stalk,color of flower,petal shape and sterility were found in MI progenies which laid a good foundation for constructing flax mutant library. It was also found that the effects of seedling rate and seed pro duetion in M1 flax were different induced by physical and chemical agents. The 60 Co-7 ray with high doses caused low seedling rate but low production, while the EMS with high concentration caused high seedling rate, but most were sterile plants which lead lower seed production.