摘要
OBJECTIVE:To study the effect of puerarin on matrix metalloproteinase-2(MMP-2)gene and protein expression in human fetal scleral fibroblasts(HFSFs)exposed to extremely low frequency electromagnetic fields(ELF-EMF).METHODS:Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h.The experimental groups were divided into subgroups treated with 0,0.1,1and 10μM puerarin respectively.The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS:MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h.Puerarin at the concentration of 0.1μM reversed this effect by 8.53%in mRNA and by 17.97%in protein expression(P<0.05).The effect was more prominent at higher concentrations(1 and 10μM,P<0.01).CONCLUSION:Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells.Puerarin reversed the action to some extent in a specific concentration range.Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.
OBJECTIVE： To study the effect of puerarin on matrix metalloproteinase-2（MMP-2） gene and protein expression in human fetal scleral fibroblasts（HFSFs） exposed to extremely low frequency electromagnetic fields（ELF-EMF）.METHODS： Cultured HFSFs were exposed to 0.2mT ELF-EMF for 24 h. The experimental groups were divided into subgroups treated with 0, 0.1, 1and 10 μM puerarin respectively. The expression of MMP-2 mRNA and protein were detected with real-time polymerase chain reaction and western-blot analysis respectively.RESULTS： MMP-2 mRNA and protein expression increased by 0.793 and 1.130 folds respectively under the exposure of ELF-EMFs at 0.2 mT flux density for24 h. Puerarin at the concentration of 0.1 μM reversed this effect by 8.53% in mRNA and by 17.97%in protein expression（P0.05）. The effect was more prominent at higher concentrations（1 and 10 μM,P0.01）.CONCLUSION： Exposure to ELF-EMFs increased the expression of MMP-2 mRNA and protein in HFSF cells. Puerarin reversed the action to some extent in a specific concentration range. Our results implied that the puerarin might protect scleral tissue from increased expression induced by exposure to ELF-EMFs.
基金
Supported by the General Program of the Bio-medical Division of the Shanghai Science and Technology Commission(10411966200)
the Scientific Research Fund of Chinese Medical of Shanghai Health Bureau(2009s023)
the Shanghai Leading Academic Discipline Project(S30205)
