肉桂总多酚的提取工艺优选及降糖作用考察

Optimization of Extraction Technology for Total Polyphenols from Cinnamomi Cortex and Investigation of its Hypoglycemic Effect

目的:优选肉桂总多酚的提取工艺条件并观察其降糖作用。方法:以肉桂总多酚提取率为指标,采用正交试验法考察乙醇体积分数、料液比、提取温度、提取时间对提取工艺的影响。以小鼠空腹血糖(FPG)为指标建立糖尿病小鼠模型,造模成功小鼠分成5组,即空白对照组、模型对照组、阳性对照组(盐酸二甲双胍)、肉桂总多酚低剂量组及肉桂总多酚高剂量组,考察肉桂总多酚对链脲佐菌素(STZ)所致糖尿病小鼠血糖的影响。结果:肉桂总多酚的最佳提取工艺参数为乙醇体积分数30%,料液比1∶10,提取时间90 min,提取温度80℃;总多酚平均提取率3.9%,总多酚质量分数39.1%。各组给药前的FPG(x珋+s,n=10)分别为(6.33±1.27),(18.06±3.12),(18.71±3.51),(18.06±3.27),(18.41±3.54)mmol·L-1,灌胃3周后则分别为(5.36±1.06),(31.59±2.94),(25.08±3.35),(15.33±4.02),(12.50±3.85)mmol·L-1。结论:优选的提取工艺科学合理,可作为肉桂总多酚中试生产的依据。肉桂总多酚具有明显降低糖尿病小鼠血糖的作用。

Objective：To optimize extraction technology of total polyphenols from Cinnamomi Cortex and observe its effect on streptozotocin （STZ）-induced diabetic mice.Method：With yield of total polyphenols as index,effects of the concentration of ethanol,solid-liquid ratio,extraction temperature and extraction time on extraction technology were investigated by orthogonal test.Diabetic mice model was established with fasting plasma glucose （FPG） as index,successful modeling mice were divided into five groups,such as the blank control group,model control group,positive control group （metformin hydrochloride）,low-dose of total polyphenols from Cinnamomi Cortex group,high-dose group,effect of total polyphenols from Cinnamomi Cortex on blood glucose of streptozotocin （STZ）-induced diabetic mice was investigated.Result：Optimum extraction process was as following：extracted 60 min with 10 times the amount of 30％ ethanol at 80 ℃ ; Average yield of total polyphenols from Cinnamomi Cortex was 3.9％,the mass fraction of it was 39.1％.Before administration,FPG of each group were （6.33 ± 1.27）,（18.06±3.12）,（18.71±3.51）,（18.06±3.27）,（18.41±3.54） mmol· L-1,respectively; While aftergavage3 w,FPH were （5.36 ±1.06）,（31.59 ±2.94）,（25.08± 3.35）,（15.33 ±4.02）,（12.50 ±3.85） mmol · L-1,respectively.Conclusion：Optimized technology was scientific and reasonable,it could be used as basis for trial production of total polyphenols from Cinnamomi Cortex.Total polyphenols from Cinnamomi Cortex had significantly decrease fasting blood glucose levels in diabetic mice.