小槐花提取物对α-葡萄糖苷酶抑制活性研究

α-Glucosidase Inhibitory Activity of Extracts from Desmodium caudatum

目的:评价小槐花Desmodium caudatum(Thunb.)DC.提取物对α-葡萄糖苷酶的抑制活性。方法:通过建立体外α-葡萄糖苷酶抑制模型,对小槐花提取物进行活性筛选。结果:小槐花提取物均有较好的α-葡萄糖酶抑制作用,其中以乙酸乙酯部位(DCEA)和石油醚部位(DCPE)活性较好(IC50=9.79,39.2 mg·L-1),其次为正丁醇部位(DCBU)(IC50=625.1 mg·L-1),抑制活性均远远大于阳性对照阿卡波糖(IC50=1 103.01 mg·L-1)。DCBU对α-葡萄糖苷酶抑制活性呈显浓度依赖性;DCPE和DCEA对α-葡萄糖苷酶抑制活性对浓度的变化较灵敏,随浓度的增加而急剧上升。结论:小槐花石油醚部位和乙酸乙酯部位对α-葡萄糖苷酶活性抑制非常显著,具有开发治疗糖尿病降血糖药物的价值。

Objective：To evaluate α-glucosidase inhibitory activity of extracts from Desmodium caudatum.Method：By establishing α-glucosidase inhibitory model in vitro,the activity of extracts from D.caudatum was screened.Result：The extracts from D.caudatum showed good inhibitory activity.The ethyl acetate extract and petroleum ether extract had higher inhibitor activity （IC50 =9.79 and 39.2 mg ·L-1）,which showed far greater activity than that of acarbose （IC50 =1103.01 mg ·L-1）,and followed by petroleum ether and n-butanol extract （IC50 =625.1 mg· L-1）.The α-glucosidase inhibitory activity of DCBU had concentration-dependent manner.α-Glucosidase inhibitory activity of DCPE and DCEA is sensitive to the change of concentration,which increased dramatically as the concentration increased.Conclusion：DCPE and DCEA showed very significant inhibitory activity to α-glucosidase,and could be exploited the diabetes drugs as α-glucosidases inhibitors in future.