摘要
目的:建立炒麦芽配方颗粒的HPLC指纹图谱分析方法。方法:采用高效液相色谱法,以Megres5-C18(Hanbon,4.6 mm×250 mm,5μm)为色谱柱,以乙腈-0.1%磷酸溶液为流动相梯度洗脱,检测波长316 nm,流速1.0 mL·min-1,柱温30℃。结果:炒麦芽配方颗粒指纹图谱共有9个共有峰,方法学考察结果良好,各批炒麦芽配方颗粒样品指纹图谱与对照指纹图谱相似度均>0.88,可以用于炒麦芽配方颗粒的定性鉴别。结论:采用HPLC指纹图谱可实现全面和整体的评价,为有效提高炒麦芽配方颗粒的质量控制提供参考。
Objective：To establish a method of fingerprint analysis on Roasted Malt Formula granules by HPLC.Method：The fingerprints chromatographic were obtained by HPLC with MegresS-C18 column （4.6 mm ×250 mm,5 μm） by gradient elution; the solvent system composed of acetonitrile-0.1％ phosphoric.The wavelength was set at 316 nm,the flow rate was 1.0 mL ·min-1 and the column temperature was maintained at 30 ℃.Result：The HPLC specific chromatogram of Roasted Malt Formula granules showed 9 common peaks.The methodological results were good.The similarity of ten batches of samples exceeded 0.88.Conclusion：The fingerprints chromatogram can realize the analysis of a formulas as a whole with supplies references for improving the quality control of Roasted Malt Formula granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第20期115-118,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2011年度江苏省企业博士聚集计划项目(2011009)
