小檗碱吴茱萸碱联用对AGS细胞TNF-α和IL-8含量的影响

Effect of Coadministration of Berberine and Evodiamine on TNF-αand IL-8 Levels in AGS Cells

目的:观察小檗碱吴茱萸碱对胃癌细胞株(AGS)细胞增殖及肿瘤坏死因子-α(TNF-α)和白细胞介素(IL-8)含量的影响。方法:采用MTT(四甲基偶氮唑盐)法测定小檗碱、吴茱萸碱及两药联用对AGS细胞生长的影响;流式细胞技术检测小檗碱、吴茱萸碱及两药联用对AGS细胞细胞周期的影响;ELISA法测定小檗碱、吴茱萸碱及两药联用对AGS细胞TNF-α和IL-8含量的影响。结果:小檗碱15μmol·L-1和吴茱萸碱45μmol·L-1联用时对AGS细胞增殖的抑制作用弱于吴茱萸碱单用;小檗碱83μmol·L-1和吴茱萸碱1.4μmol·L-1联用时对AGS细胞增殖的抑制作用稍优于两药单用,两药联用阻滞细胞周期于G2/M期的作用较两药单用无显著差异。进一步研究发现,小檗碱83μmol·L-1和吴茱萸碱1.4μmol·L-1联用增加AGS细胞培养上清肿瘤坏死因子-α(TNF-α)含量的作用优于两药单用。小檗碱具有降低AGS细胞培养上清IL-8含量的趋势,而吴茱萸碱能够显著增强AGS细胞培养上清IL-8的含量(P<0.01),两药联用能够显著降低AGS细胞培养上清中IL-8的含量(P<0.01)。结论:不同剂量的小檗碱吴茱萸碱联用对AGS细胞增殖的影响不同。小檗碱能够抑制吴茱萸碱诱导的AGS细胞IL-8的表达上调。

Objective：To investigate the effects of coadministration of berberine and evodiamine on cell growth,tumor necrosis factor-α （TNF-α） and interleukin （IL）-8 levels of gastric cancer cells （AGS） cells.Method：The inhibitory effect of coadministration of berberine and evodiamine on AGS cell growth was measured by MTT method.Cell cycle distributions were measured by flow cytometry.TNF-α and IL-8 contents were measured by ELISA method.Result：Coadministration of berberine （15 μmol ·L-1） and evodiamine （45 μmol ·L-1） triggered weaker inhibitory effect on the proliferation of AGS cells than that achieved by evodiamine alone,coadministration of berberine （83 μmol ·L-1） and evodiamine （1.4 μmol ·L-1） obtained slightly better inhibitory effect on the proliferation of AGS cells than that of berberine or evodiamine alone,however,their co-administration did not obtain better cell cycle arrest than berberine or evodiamine alone.Further studies showed that,coadministration of berberine （83 μmol ·L-1） and evodiamine （1.4 μmol ·L-1） induced the increase of TNF-α level in AGS cell culture supernatant which was superior to that triggered by usage of the two drugs alone.Berberine reduced IL-8 contents in the AGS cell culture supernatant but without significance difference,while,evodiamine could significantly enhance the IL-8 contents in AGS cells culture supernatant.Then their coadministration could significantly reduce the IL-8 levels in the AGS cell culture supernatant.Conclusion：Different doses of berberine and evodiamine combination can result in different effect on the proliferation of AGS cells.And berberine could inhibit the increase of IL-8 induced by evodiamine.