补肾中药对雷公藤多苷所致雄性幼鼠生殖损伤的保护作用及最终生育能力的影响

Juvenile Male Rats Reproductive Damage by Glycosides of Tripterygium wilfordii and Its Protection Role Bushen Chinese Herbs and Observition Finals Fertility of Male Rats

目的：探讨雷公藤多苷（glycosides of Tripterygium wilfordii,GTW）对雄性幼鼠生殖损伤和损伤后最终生育能力的影响及补肾中药（菟丝子黄酮、六味地黄丸）的保护作用.方法：①生殖损伤研究：3～4周龄SD雄性幼鼠48只,随机分为4组：空白组（CMC-Na）、雷公藤多苷组（9 mg·kg-1·d-1）单独ig,六味组（六味地黄丸1 g·kg-1 ·d-1＋ GTW 9 mg·kg-1·d-1）,黄酮组（菟丝子黄酮0.1 g·kg-1·d-1 ＋GTW 9 mg·kg-1·d-1）混合ig,持续12周后,处死取一侧睾丸、附睾立即称质量,计算脏器指数,称重后放入固定液中保存,待观察睾丸组织形态学改变；②生育能力研究：成年大鼠100只,每组25只,分组喂养方法同上,持续12周后,将所有大鼠分笼喂养,每笼1只,按照1∶1比例放入成年雌性大鼠1只,分3次合笼,每次合笼时间为15 d,合笼后观察雌鼠受孕率、仔鼠离乳存活率、仔鼠生长发育情况.结果：雷公藤多苷对睾丸病理组织生精细胞肿胀变性,精原细胞、精母细胞、精子细胞明显减少,菟丝子黄酮、六味地黄丸能明显减轻这种损伤；用药12周后雄性幼鼠体重、睾丸质量、睾丸指数、附睾质量、附睾指数与空白组相比无显著性差异（P＞0.05）；每次合笼后受孕率、离乳存活率、幼鼠的生长发育与空白组相比无显著性差异.结论：雷公藤多苷对雄性幼鼠生殖系统的损伤是可逆的,对睾丸的病理组织损伤是可以恢复的,补肾中药具有保护这种病理组织损伤作用；雷公藤多苷对最终的生育能力没有影响,对幼仔生长发育没有影响.

Objective：The research that the juvenile male rats reproductive damage and injury fertility was impacted by glycosides of Tripterygium wilfordii （GWP） and its protection role Bushen Chinese herbs （Cuscutae Semen flavone,Liuwei Dihuang wan）.Method：The first part of reproductive damage and histopathological study group：3-4 week-old SD 48 male rats were randomly divided into 4 groups：control group,GTW,Liuwei Dihuang Wan group,Cuscutae Semen flavone group.After 12 weeks,rats were sacrificed,testicle,epididymis immediately weighed to calculate the viscera index,weighed and placed in fixative save,morphological changes of testicular tissue to be seen; part Ⅱ：cage all rats fed one per cage,an adult female rats were placed in accordance with the ratio of 1∶ 1.Three times in the same cage,each co-cage time is 15 days,the pregnancy rate of mated females observed after pups weaning survival,growth and development of offspring.Result：GTW can make testicular germ cell swelling and degeneration,can make spermatogonia,spermatocytes,sperm cells decreased significantly.Cuscutae Semen flavone,Liuwei Dihuang wan can significantly reduce this damage; after 12 weeks with GWP fed male pups：weight,testicular weight,testicular index,epididymal weight,epididymal index indicators blank group comparison showed no significant difference.Each mated conception rates,weaning survival,growth and development of the pups in each group comparison showed no significant difference.Conclusion：GTW damage to the reproductive system is reversible; pathological damage to reproductive organs can be restored ; Bushen Chinese herbs can fight TWP caused reproductive damage; no effect on the fertility of the late cubs growth and development is not impact.