酶法提取甘薯膳食纤维的研究

Study on extraction of dietary fiber from sweet potato by enzymatic hydrolysis

利用液化酶、糖化酶及木瓜蛋白酶水解甘薯渣,提取膳食纤维,并对膳食纤维产品的成分组成和性能进行了分析。结果显示,甘薯膳食纤维的最佳提取条件为:0.2mol/L NaOH浸泡薯渣1h,400u/mL液化酶70℃酶解40min,600u/mL糖化酶60℃酶解50min,400u/mL木瓜蛋白酶35℃酶解40min。此条件下,甘薯膳食纤维的最高提取率为268mg/g。提取得到的甘薯膳食纤维中有效成分含量达到72.45%,水分含量为10.07%。甘薯膳食纤维的持水性高达16.76g/g,吸水膨胀性为21.07mL/g,明显高于其他膳食纤维,说明提取的产品在食品加工中具有良好的发展前景。

The dietary fiber was extracted,using liquefied enzyme,saccharifying enzyme and enzyme papain hydrolysis from sweet potato residue.The composition and functional properties of dietary fiber products were analyzed.Resuits showed the best extraction conditions were that 0.2mol/L NaOH soak the potato residue for 1h,400 u/mL liquefied enzyme enzymolysis for 40 minnutes on 70 ℃,600 u/mL saccharifying enzyme enzymolysis for 50 minutes at 60 ℃,400 u/mL papain enzymolysis for 40minutes at 35 ℃.Under these conditions,the highest extraction yield of sweet potato dietary fiber was 268 mg/g.The effective component content of dietary fiber products was 72.45％,and the moisture content of that was 10.07％.The water binding capacity of the sweet potato dietary fiber reached to16.76 g/g,water absorption expansion was 21.07 mL/g,which was significantly higher than other dietary fiber.The extraction has good development prospect in food processing.