枸杞总黄酮的提取及抗氧化作用研究

Study on the extraction method and antioxidation ability of flavonoids in Lycium barbarum L.

目的:研究枸杞总黄酮的提取方法,并比较不同方法所得提取物抗氧化作用的强弱。方法:分别以回流提取法和超声提取法提取宁夏枸杞中的黄酮类物质,以提取液总黄酮含量为指标,通过正交试验优选其提取条件,并将不同方法所得提取物对油脂的的抗氧化作用进行比较。结果:超声提取法对总黄酮的提取量高于回流提取法,且超声提取物对油脂的抗氧化作用也较强。结论:超声提取法更有利于枸杞总黄酮的提取及抗氧化活性的保持。

Objective to study the methods of extracting flavonoids in Chinese wolfberry,and compare the antioxidant effect from different extraction method s.Methods reflux extraction and ultrasonic extraction were used to extract flavonoids in Lycium barbarum L.respectively.An orthogonal experiment was designed to optimize the extraction conditions.Antioxidant effect of different extraction were examined and compared by examining POV from each extracts.Results the extraction ratio of flavonoids by ultrasonic extraction was higher than the extraction ratio by reflux extraction.Oxidation effect of the extract by ultrasonic was stronger.Conclusion ultrasonic extraction was the better means to extract flavonoids from Lycium barbarum L.