粒毛盘菌突变株油脂GC-MS分析及其亚油酸甲酯的纯化

Purification of methyl linoleate and analysis of oils produced by Lachnum mutant strain

亚油酸甲酯具有较强的触杀、产卵抑制和驱避作用。对一株粒毛盘菌产油脂突变株Z60胞外油脂进行了GC-MS分析,其中亚油酸甲酯(C18:2)占总脂肪酸甲酯质量分数的32.7%。以亚油酸甲酯含量为指标,在单因素基础上,采用正交实验对亚油酸甲酯的纯化条件进行了优化,最佳条件为3.0 g粒毛盘菌胞外油脂,6.6 g尿素,16.5 mL甲醇溶液,-5℃包合18 h,亚油酸甲酯纯度为50.1%。进一步利用硝酸银薄层层析法对其进行分离,分离后亚油酸甲酯气相色谱纯度提高到96.7%。

Methyl linoleate has strong bioactivity of touch kill, oviposit inhibition and repellency. Gas chromatography-mass spectrometry analysis of extracellular lipids produced by Lachnum mutant strain Z60 was performed and the results indicated that the content methyl linoleate was 32.7%. Based on the single factor experiment, the conditions of purification of methyl linoleate were optimized by orthogonal test. The results were as follows： fatty oils 3.0 g, urea 6.6 g, methnol 16.5 mL, -5℃ inclus 18 h. Under this condition, the purity of methyl linoleate reached 50.1%. Furthermore, separation of methyl linoleate linoleate reached 50.1%. Furthermore, separation of methyl linoleate was up to 96.7%.