胰蛋白酶酶解法制备蛤蟆油蛋白工艺的研究

Preparation technology of proteins from Oviductus Ranae by trypsin hydrolysis

目的:建立胰蛋白酶酶解法制备蛤蟆油蛋白的最佳工艺。方法:采用单因素和正交试验对蛤蟆油蛋白提取的条件进行优化,高效液相凝胶色谱法考察蛤蟆油蛋白的酶解条件,优选出制备蛤蟆油蛋白的最佳工艺。结果:最佳工艺条件为柠檬酸pH4.5,料液比为1:100(g/mL),浸提时间为12 h,胰蛋白酶与底物比为1:100(g/g),酶解时间为12 h。结论:验证试验结果表明,蛤蟆油蛋白提取率为80.66%,蛋白含量为45.95%。

Objective： To establish the optimal preparation technology Oviductus Ranae protein by using trypsin. Methods： Monofactor test and the orthogonal experiment were developed to determine the optimum extraction process, investigate the trypsin hydrolyze conditions by high performance liquid gel permeation chromatography. Results： The optimum preparation technology was obtained as follows： citric acid pH was 4.5, material/liquid ratio was 1 g：100 mL, extracted 12 h, trypsin/substrate ratio was 1 g：100 g, hydrolyzed 12 h. Conclusion： Verifying test results show that the Oviductus Ranae protein yield was 80.66%, protein content was 45.95%.