小叶莲化学指纹图谱研究

Research on chemical fingerprint chromatograms of Sinopodophyllum hexandrum

目的:研究小叶莲的化学成分指纹图谱,评价小叶莲商品药材质量,并比较桃儿七不同药用部分之间的主要成分差异,为临床安全、合理应用提供依据。方法:采用小叶莲HPLC指纹图谱方法,测定了12份小叶莲的指纹图谱,确定了20个色谱峰作为共有峰,并指认了其中12个色谱峰;运用该方法比较了桃儿七根及根茎与小叶莲指纹图谱的差异,通过相似度评价和聚类分析对图谱数据进行分析。结果:本研究首次报道了小叶莲中4个黄酮类成分;首次指认了小叶莲指纹图谱中11个色谱峰,包括9个黄酮和2个木脂素类成分;桃儿七不同药用部分化学成分存在较明显的差异,小叶莲主要含异戊烯基黄酮类成分,桃儿七根和根茎主要含鬼臼毒素和简单取代的黄酮及其苷类成分。结论:该指纹图谱方法可以作为评价比较小叶莲、桃儿七根及根茎中黄酮类和木脂素类成分的相对含量及其质量的依据。同时,为小叶莲、桃儿七根及根茎不同药用部分的药效学差异研究提供参考。

Objective： To study the HPLC fingerprint of Sinopodophylli Fructus, compare the major chemical differences in fruit of Sinopodophyllum hexandrum as well as the roots and rhizomas of S. hexandrum, and provide scientific evidence for clinical ap- plication and quality control. Method： HPLC fingerprint method was used to analyze 12 fruits of S. hexandrum. A total of 20 common peaks were confirmed, and 12 peaks in the HPLC fingerprint were identified. Furthermore, similarity evaluation method and clustering analysis method were introduced to compare HPLC chromatograms, between fruits and underground parts. Result： In this paper, quer- cetin-3-O-fl-D-glucopyranosid, kaempferol-3-O-fl-D-glucopyranoside, 8-prenylquercetin and 8-prenylquercetin 3-methyl ether were firstly reported in Sinopodophylli Fructus. Among the existing fingerprint research, a total of 11 peaks were identified for the first time, containing 9 flavonoids and 2 lignans. The chemical constituents differed significantly in different medicinal parts of S. hexandrum. Prenylflavonoid compounds were the main constituents of Sinopodophylli Fructus. However, podophyllotoxin, flavonoids with simple substituent groups and flavonoid glycosides were the major ingredients in the roots and rhizomas of S. hexandrum. Conclusion： This method can be used for the quality control of Sinopodophylli Fructus and the roots and rhizomas of S. hexandrum. It has provided a ref- erence for the pharmacodynamic differences of the two different parts.