小鼠原位肝癌移植模型中髓系来源抑制性细胞的表达

The expression of myeloid-derived suppressor cells in an orthotopic transplantation liver tumor model in mice

目的 观察小鼠原位肝癌移植模型中髓系来源抑制性细胞（MDSCs）的表达.方法 将10只BALB/c小鼠随机分为两组：正常组和荷瘤组,后组是将H22肝癌细胞注射到肝左外叶,制作小鼠原位肝癌移植模型.10d后处死小鼠.流式细胞术检测两组小鼠外周血、骨髓、脾脏及肝脏组织中MDSCs的表达.结果 荷瘤组小鼠外周血、骨髓和脾脏中的MDSCs比例为[（47.73±6.00）、（71.90±4.30）、（11.21±1.19）％],均明显高于正常组小鼠[（18.33±2.31）、（59.03±4.50）、（5.82±0.58）％]；正常小鼠肝组织中MDSCs占肝内白细胞6.5％,荷瘤小鼠肝癌组织、癌旁组织中MDSCs占肝内白细胞分别为43.8％和12.8％.结论 在小鼠原位肝癌移植模型中,MDSCs表达明显上调,为研究MDSCs在肝癌发生发展中的作用提供了良好的动物模型.

Objective To investigate the expression of myeloid-derived suppressor cells （MDSCs）in an orthotopic transplantation liver tumour model in BALB/c mice.Methods Ten healthy BALB/c mice were randomly divided into two groups （n =5 each）：normal group and tumor-bearing group.The murine hepatic cancer cell line H22 cells were transplanted into the left liver lobe of mice to establish an orthotopic transplantation liver tumor model.The mice were sacrificed at 10th day.The expression of MDSCs in peripheral blood,bone marrow,spleen and liver was analyzed by flow cytometry.Results The presence of CD11b＋ Gr-1 ＋ MDSCs was significantly increased in the peripheral blood,bone marrow,and spleen of tumor-bearing mice [（47.73 ±.6.00）,（71.90 ±4.30）,（11.21 ± 1.19）％] as compared with normal mice [（18.33 ±2.31）,（59.03 ±4.50）,（5.82 ±0.58）％].The percentage of CD11b＋ Gr-1 ＋ MDSCs of intrahepatic leukocytes in normal mice was 6.5％.However,the percentage of MDSCs of intrahepatic leukocytes in tumor and paracancerous tissue was up to 43.8％ and 12.8％ respectively.Conclusion MDSCs were elevated in an orthotopic transplantation tumor mouse model of hepatocellular carcinoma.The mouse model can serve to study the role of MDSCs in hepatocellular carcinoma development.