罗格列酮对肝星状细胞基质金属蛋白酶-2和-9基因表达的影响

Effects of rosiglitazone on expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in hepatic stellate cells

目的 观察过氧化物酶体增殖物活化受体γ（PPARγ）特异配体罗格列酮对肝星状细胞（HSC）表达基质金属蛋白酶（MMP）-2和MMP-9的影响.方法 在培养的HSC株中加入不同浓度的罗格列酮（终质量浓度为5、10、15、20 μmol/L）,于24 h后收取细胞,利用半定量逆转录-聚合酶链反应（RT-PCR）测定MMP-2、MMP-9 mRNA表达.结果 MMP-2表达水平在罗格列酮5、10、15、20 μmol/L组分别为0.5708±0.0609、0.8900±0.0823、1.1348±0.1205、1.4490±0.0832,而空白对照组为0.3237±0.0796.MMP-9表达水平在罗格列酮5、10、15、20 μmol/L组分别为0.5487±0.0770、0.7554±0.0510、0.9497±0.0451、1.1088±0.0777,空白对照组为0.3220±0.0592.结论 罗格列酮可增强HSC对MMP-2、MMP-9的表达,并在一定范围内,呈剂量依赖性.

Objective To investigate the effects of rosiglitazone,a ligand of peroxisome proliferators activated receptor gamma （PPAR-γ）,on the expression of matrix metalloproteinase （MMP）-2 and MMP-9 in hepatic stellate cells （HSCs）.Methods HSCs were cultured in medium containing different concentrations of rosiglitazone （5,10,15,20 μmol/L respectively）,and harvested at 24 h.The expression of MMP-2 and MMP-9 was detected by using reverse transcription polymerase chain reaction.Results The MMP-2 expression in HSCs cultured with different concentrations of rosiglitazone （5,10,15,20 μmol/L）was 0.5708 ±0.0609,0.8900 ±0.0823,1.1348 ±0.1205 and 1.4490 ±0.0832 respectively,and it in control group was 0.3237 ± 0.0796.The MMP-9 expression in HSCs cultured with different concentrations of rosiglitazone （5,10,15,20 μmol/L） was 0.5487 ±0.0770,0.7554 ±0.0510,0.9497 ±0.0451 and 1.1088 ±0.0777 respectively,and it in control group was 0.3220 ±0.0592.Conclusion PPAR-γ ligand rosiglitazone may up-regulate the expression of MMP-2 and MMP-9 in HSCs dose-dependently to some extent.