赖氨酰氧化酶样蛋白-2对胰腺癌细胞的影响

Effects of silencing lysyl oxidase-like 2 protein expression on human pancreatic cancer cells

目的 观察沉默赖氨酰氧化酶样蛋白-2（LOXL2）基因对胰腺癌细胞的影响.方法 利用脂质体法将构建好的LOXL2-短发卡RNA（shRNA）质粒转染到胰腺癌Panc-1细胞中,应用Western blot检测转染前后胰腺癌Panc-1细胞中LOXL2蛋白表达量的变化,Transwell侵袭实验检测胰腺癌细胞的体外侵袭力,MTT法检测胰腺癌细胞的黏附能力.结果 LOXL2-shRNA转染组Panc-1细胞中LOXL2蛋白表达水平（0.32±0.01）、体外侵袭力[（32.2±3.8）个]、黏附率[（75.4±3.2）％]较转染前[1.05±0.05、68.6±3.5、（90.3±2.1）％,P＜0.05]明显降低.结论 沉默LOXL2基因能抑制胰腺癌Panc-1细胞的侵袭能力和黏附能力.

Objective To investigate the effects of silencing lysyl oxidase-like 2 protein （LOXL2）gene on pancreatic cancer cells.Methods LOXL2-short hairpin RNA （shRNA） plasmid was constructed by using lipofectamine,and transfected into pancreatic cancer Panc-1 cells.The changes of LOXL2 protein expression in pancreatic cancer panc-1 cells before and after transfection with LOXL2-shRNA were assayed by using Western blotting.Transwell invasion assay was used to assess the in vitro invasive ability of pancreatic cancer cells.Adhesive capacity of pancreatic cancer cells was measured by methyl thiazol tetrazolium （MTT） assay.Results The grey-scale value of LOXL2 protein in Panc-1 cells in LOXL2-shRNA transfection group was （0.32 ± 0.01）,the number of migrating cells was （32.2 ± 3.8） and adhesive capacity was （75.4 ± 3.2）％,which were significantly reduced as compared with those before transfection [1.05 ± O.05,68.6 ± 3.5,and （90.3 ± 2.1） ％,P ＜ 0.05].Conclusion Silencing LOXL2 gene can inhibit the invasive ability and adhensive capacity of pancreatic cancer Panc-1 cells.