转移性人肝癌裸小鼠原位种植合并姑息性射频消融模型的建立

Establishment of metastatic human hepatocellular carcinoma orthotopic transplantation model in combination with palliative radiofrequency ablation in nude mice

目的 建立稳定有效的转移性人肝癌裸小鼠原位种植合并姑息性射频消融模型.方法 采用60只BALB/c-nu/nu裸小鼠,建立转移性人肝癌裸小鼠原位种植模型；随机分为两组,移植瘤术后30 d进行姑息性射频消融术和仅开腹（假手术组）.两组中各随机抽出7只裸鼠,在2次手术后4周处死裸鼠,观察肝脏移植瘤大小、坏死范围并行免疫组织化学染色检测血管内皮生长因子（VEGF）和基质金属蛋白酶（MMP）-2蛋白的表达.结果 人肝癌裸小鼠原位种植成瘤率为100.0％ （60/60）；姑息性射频消融成功率为96.7％ （29/30）；与假手术组比较,姑息性射频消融组移植瘤组织VEGF和MMP-2蛋白表达降低（P＜0.01）.结论 转移性人肝癌裸小鼠原位种植合并姑息性射频消融模型手术成功率高,移植瘤组织VEGF和MMP-2蛋白表达降低.

Objective To establish the metastatic model of human hepatocelular carcinoma （HCC） in nude mice via orthotopic transplantation and further to palliative radiofrequency ablation （RFA） so as to provide a practicable model for the study of biological effects of RFA.Methods Human HCC cell line MHCC97-L was inoculated into 60 BALB/c nude mice.All these cases were divided equally into palliative RFA and sham-operation subgroups.Four weeks after the second operation,the tumor volumes,necrosis range and immunohistochemical staining of vascular endothelial growth factor （VEGF） and matrix metalloproteinase （MMP）-2 were measured in 7 randomly selected nude mice of each subgroup.Results All nude mice with xenograft tumors exhibited 100.0％ （60/60） transplantability,and 96.7％ （29/30） palliative RFA successful rate was achieved.The expression levels of VEGF and MMP-2 were significantly lower in palliative RFA group than in sham operation group （P ＜ 0.01）.Conclusion Metastatic human hepatocellular carcinoma orthotopic transplantation model in combination with palliative RFA in nude mice has a high success rate.After orthotopie tumor transplantation,palliative RFA could reduce the expression of VEGF and MMP-2.