可逆识别Hg^(2+)的罗丹明基荧光增强型探针及在活细胞成像中的应用

A Reversible Fluorescence Enhancement Probe for Hg^(2+) Based on Rhodamine and Its Application in Live Cell Imaging

基于罗丹明类荧光团,合成了一个新型的Hg2+荧光探针RS.通过紫外光谱法和荧光光谱法研究了探针RS在EtOH-HEPES缓冲液(V∶V=1∶1,pH=7.3)中对Hg2+的识别特性.结果表明:探针RS可快速、可逆识别Hg2+.随着Hg2+的滴加,探针RS的内酰胺环结构发生开环,并形成1∶1 Hg2+配合物.在L929活细胞中的荧光显微成像表明RS可应用于生物体的检测.

A novel of Hg2＋ fluorescence probe （RS） with rhodamine chromophore was synthesized, and its structure was characterized by 1H NMR, MS spectra and elemental analysis. The recognition properties of the probe RS with Hg2＋ ions had been investigated in EtOH-HEPES buffer solution （V： V= 1 ：1, pH-7.3） by the ultraviolet spectrophotometry and the fluo- rescence spectrophotometry. The results showed that the recognition is instantaneous and reversible. Upon the addition of Hg2＋, the spirolactam ring of probe RS was opened and 1 ： 1 ligand-mercury complex was formed. The fluorescence imaging experiments of Hg2＋ in L929 living cells demonstrated that the probe RS could be used in biological systems,