摘要
基于罗丹明类荧光团,合成了一个新型的Hg2+荧光探针RS.通过紫外光谱法和荧光光谱法研究了探针RS在EtOH-HEPES缓冲液(V∶V=1∶1,pH=7.3)中对Hg2+的识别特性.结果表明:探针RS可快速、可逆识别Hg2+.随着Hg2+的滴加,探针RS的内酰胺环结构发生开环,并形成1∶1 Hg2+配合物.在L929活细胞中的荧光显微成像表明RS可应用于生物体的检测.
A novel of Hg2+ fluorescence probe (RS) with rhodamine chromophore was synthesized, and its structure was characterized by 1H NMR, MS spectra and elemental analysis. The recognition properties of the probe RS with Hg2+ ions had been investigated in EtOH-HEPES buffer solution (V: V= 1 :1, pH-7.3) by the ultraviolet spectrophotometry and the fluo- rescence spectrophotometry. The results showed that the recognition is instantaneous and reversible. Upon the addition of Hg2+, the spirolactam ring of probe RS was opened and 1 : 1 ligand-mercury complex was formed. The fluorescence imaging experiments of Hg2+ in L929 living cells demonstrated that the probe RS could be used in biological systems,
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
2013年第10期2210-2215,共6页
Chinese Journal of Organic Chemistry
基金
四川省科技厅应用基础(No.2009JY0078)
四川省教育厅重点(No.11ZA154)
乐山市科技局重点(No.2011SZD107)资助项目~~