3Sierkstra L N, Klugkist J, Markvardsen P, et aL Protease variants and coropositions[P}. US 20110230386 A1, 2011-09-22.
4Acevedo J, Rodriguez V, Saavedra M, et al. Cloning, expression and decoding of the cold - adaptation of a new widely represented thermolabile subtilisin- -like protease[J]. Journal of Applied Microbiology, 2013, 114(2): 352-363.
5Stmu.sberg S L, Ruan B, Fisher K E, et al. Directed coevolution of stability and catalytic activity in calcium-free subtilisin[J]. Biochemistry, 2005. 44: 3272-3279.
6Li Z, Roccatano D, Lorenz M, et al. Directed evolution of Subtilisin into a highly active and guanidiniuro chloride - and sodium dodecyl.sulfate- -tolerant protease[J]. Chem Bio Chem, 2012, 13: 691-699.
7Martinez tk, Jakob F, Tu R, et al. Increasing activity and them,al resistance of bacillus gibsonii alkaline protease (BgAP) by directed evolutionU]. Biotechnology and Bioengineering, 2013, 110(3) :711-720.
9Schroeder M, Lenting H B M, Kandelbauer A, et al. Restricting detergent protea.se acdon to surface of protein fibres by chemical modification[J]. Applied Microbiology and Biotechnology, 2006, 72: 738-744.
10Nielsen L K, Deane-wray A. 4-substituted- phenyl-boronic acids as enzyme stabilizet[PJ. EP 0832174 B1,2002-05-08.
9Khajeh K, Ranjbar B, Naderi-Manesh H, Habibi A E,Nemat-Gorgani M. Chemical modification of bacterial α-amylases; changes in tertiary structures and the effect of additional cacium[J]. Biochim Biophys Acta,2001,1548:229-237.
10Brugger R, Kronenberger A, Bischoff A, Hug D, Lehmann M, van Loon APGM, et al. Thermostability engineering of fungal phytases using low-Mr additives and chemical crosslinking[J]. Biocatal Biotransform,2001,19:505-516.