肝脏疾病与HBV前C区1896位点突变的研究

The Relationship of Point Mutation of Site 1896 of HBV Precore with Liver Diseases

目的 :分析乙型肝炎病毒 ( HBV)前 C区 1 896位点突变与慢性乙型肝炎 ( CAH)、活动性肝硬化( ALH)和肝细胞肿瘤 ( HCC)的关系。方法 :根据 HBV前 C区 DNA序列 ,采用聚合酶链式反应 ( PCR)分析 HBV第 1 896位核苷酸 G→ A( A1 896)的突变。结果 :通过对三种肝脏疾病患者血清的检测 ,发现CAH、ALH和 HCC患者血清中均存在 HBV突变株 ,其阳性率分别为 47.2 2 % ( 1 7/36)、53.33% ( 9/1 5)和 2 6.0 8% ( 6/2 3)。结论 :CAH和 ALH患者检出率较高 ,而 HCC患者检出率较低 ,说明 HBV突变与HBV在人体持续感染及感染后病情恶化有关 ;HBV突变是引起 HCC的原因之一 。

Objective To investigate the relationship of the site 1896 point mutation of HBV precore with chronic hepatitis,active cirrhosis and hepatocellular carcinoma(HCC).Methods According to the precore region of HBV.Polymerase chain reaction (PCR) is used to investigate the point mutation of site 1896 of HBV(G→A).Results Through the examination of the HBV site 1896 in the serum in these patients,find that the point mutation exists in all these types of patients,and the positive rates are 47 22%(17/36),53 33%(9/15)and 26 08%(6/23)respectively.Conclusion The positive rates of HBV site 1896 point mutation are relatively higher in CAH and ALH than that in HCC patients.which indicates that the mutation of HBV has relationship with the long term of HBV infection and the deterioration of liver damage by HBV.And the mutation of HBV is one of the inducing factors of HCC,but not the major factor.