诱导纤维堆囊菌高产埃博霉素的种间微生物筛选及鉴定

Screening and Identification of Interspecies Microorganisms Inducing Overproduction of Epothiones in Sorangium cellulosum

微生物之间存在相互作用,引入异己微生物通常可以诱导某微生物的代谢调控。筛选到一株能促进纤维堆囊菌高产埃博霉素的微生物,鉴定为烟曲霉Aspergillus fumigatusM03。该菌株的有机粗提物可以诱导纤维堆囊菌埃博霉素A和B的产量分别提高72.3%和59.7%,其适量菌体水相粗提物也有相同的诱导作用,使埃博霉素A和B的产量分别提高25.6%和24.7%。该菌株的菌体水相粗提物高温失活后对埃博霉素合成的诱导作用消失,添加浓度增加时出现抑制作用。该菌株发酵液对埃博霉素的合成没有促进作用,且随着浓度的增加抑制作用增强。

Introducing other microorganisms generally can induce microorganisms to regulate their metabolism which is based on interspecies interactions. This research screened a strain of fungus named as Aspergillus fumigatus M03 that can induce Sorangium cellulosum to overproduce epthilones. The yields of epothilone A and B can be enhanced 72.3% and 59.7% respectively by the organic crude extracts from A.fumigatus M03. Appropriate amount of its intracellular aqueous crude extracts also can improve the yields of epothilone A and B each by 25.6% and 24.7%, whereas the capability of improving disappears when the crude extracts are dealt with high-temperature, and inhibition of epothilones biosynthesis will show when the concentration of the crude extracts is high in fermentation broth of Sorangium cellulosum. The fermentation broth of A. fumigatus M03 does not promote biosyntheses of epothilones, but tends to inhibit them as the concentration increases.