西沙野生诺尼种子内生细菌群落多样性的初步研究

Investigation on Diversity of Endophytic Bacterial Community in Xisha Wild Noni(Morinda citrifolia L.)Seed

通过构建16S rDNA克隆文库的非培养方法对我国西沙野生诺尼种子内生细菌群落结构多样性进行研究,共涉及Proteobacteria、Firmicutes、Bacteroidetes及Actinobacteria四大类群,包含33个属,42个OTU,优势菌属(丰度)依次为Enterobactersp.(17.55%)、Bacillussp.(16.79%)、Acinetobactersp.(6.11%)、Pseudomonassp.(6.11%)及Piscinibactersp.(6.11%)。这是国内外首次利用非培养方法针对诺尼种子内生细菌群落多样性进行研究的报道。

Using the 16S rDNA clone library technique, a priliminary study was conducted on the community diversity of endophytic bacteria in seeds of Xisha wild Noni. The results indicated that the endophytic bacteria] communities contained 42 Operational Taxonomic Units （ OTUs ） in their clone libraries respectively, involving 33 genus from 4 groups of Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. The dominant bacterium （ abundance ） were Enterobacter sp. （ 17.55% ） , Bacillus sp. （ 16.79% ） , Acinetobacter sp. （ 6.11% ） , Pseudomonas sp. （ 6.11% ） and Piscinibacter sp. （ 6.11% ） . This was the first report of the study on endophytic bacterial communities in the Noni seeds with culture-independent method.