灵芝总甾醇的闪式提取工艺优化及其抗氧化活性

Study on sterols extraction with high-speed shearing and antioxidation in ganoderma

采用高速剪切闪式提取技术从灵芝中提取总甾醇。GC-MS分析表明:灵芝总甾醇提取物中主要含有麦角甾醇和麦角甾-7,22-二烯-3β-醇。通过单因素实验和响应面实验优化提取工艺,在剪切机转速8 400 r/min、液料比25∶1(mL∶g)、剪切时间9 min的最优条件下,麦角甾醇实际得率为0.072 1%。对灵芝总甾醇提取物进行了抗氧化活性的测定,V C做阳性对照,灵芝总甾醇(以麦角甾醇计)浓度为1mg/mL时,对DPPH·的清除率达95.16%,对ABTS+·的清除率达81.33%,对·OH的清除率达55.41%,还原力吸光度为1.746。

Extract sterol in Ganoderma by shear emulsifier was studied. Ergosterol and ergosta-7,22-dien-313-ol were extracted by GC-MS. On the basis of single factor experiments, response surface methodology was employed to optimize the extraction conditions. The ergosterol yield was 0. 721‰ under the optimal condition （revolving speed of 8400r/min, liquid-to-material ratio of 25mL/g,time of 9min）. The antioxidative activity of total sterol was compared with VC. When the concentration of total sterol reached to 1 000 μg/mL, the scavenging to DPPH · reached 95. 16% , the scavenging to ABTS^＋ · was 81.33% , the scanvenging to · OH reached 55.41% , the reducing power absorbance was 1. 746.