基于小角X射线散射技术研究再生丝素蛋白体系的方法

SAXS Method Used for Regenerated Silk Fibroin Based Materials

小角X射线散射(SAXS)技术在研究高分子材料的亚微观结构(数纳米至数百纳米)方面具有独特的优势,是研究蛋白质结构的一种重要手段。再生丝素蛋白(RSF)不仅具有优异的生物相容性,而且还可以应用于仿生纺丝研究,在生物医用材料及仿生材料领域具有广泛的应用前景。RSF材料一般由溶液成型为薄膜、纤维等固态材料,为了改善RSF材料的性能,有必要了解其成型前后的微观、亚微观结构。本文提出了高浓度RSF溶液的SAXS研究方法,得到了溶液中丝素蛋白的聚集态结构参数,构建了其三维结构模型;对RSF薄膜采用Debye-Bueche方法进行分析,根据相关函数得到了其结构信息;对RSF纤维采用Ruland方法得到了纤维取向、界面及相关长度等信息。

Small Angle X-ray scattering （SAXS） is a universal technique to study the submicroscopic structures of polymers including proteins. As one of the most available biomaterials, regenerated silk fibroin （RSF） with outstanding biocompatihilities has great potential applications in biomedical materials as well as biomimetie materials. RSF materials, such as film and fiber, are normally processed from its solution. To improve the properties of RSF materials, it is necessary to understand the microscopic/submicroscopic structures of RSF before and after processing. This paper introduced SAXS methods for concentrated RSF solution to obtain the aggregation structure parameters and construct the 3D model of RSF solution. The structure parameters of RSF film were obtained from correlation function by using Debye-Bueche method. The long period, interface and orientation of RSF fibers were calculated by applying Ruland method.