固相萃取-分散液液微萃取-高效液相色谱法测定椰子汁中酸性植物激素

Determination of Acidic Phytohormones in Natural Coconut Juice by Combining Solid-phase Extraction and Molecular Complex-based Dispersive Liquid-liquid Microextraction with High Performance Liquid Chromatography

本文通过固相萃取结合分子络合物-分散液液微萃取,与高效液相色谱联用,建立了一种测定椰子汁中酸性植物激素的新方法。选择了几种典型酸性植物激素吲哚乙酸、水杨酸、脱落酸和吲哚丁酸作为分析物,考察了该方法的萃取性能。在固相萃取与分子络合物-分散液液微萃取联用模式中,椰子汁中分析物首先吸附在C18萃取材料上,待解吸完成后,解吸液又可用作分散液液微萃取的分散剂,大大简化了萃取步骤。该方法的富集倍数可达319～478倍,线性关系良好,具有良好的精密度和准确度,有望用于植物激素的检测。

A new method combining solid-phase extraction（SPE） and molecular complex-based dispersive liquid-liquid microextraction（MC-DLLME） with high performance liquid chromatography（HPLC） has been developed for the determination of several acidic phytohormones in natural coconut juice. Indole-3- acetic acid （IAA）, salicylic acid （SA）, abscisic acid （ABA） and indolyl-3-butyricacid （IBA） were selected to investigate the extraction performance and the method sensitivity. In solid phase extraction-dispersive liquid-liquid microextraction（SPE-DLLME） procedure, analytes were firstly adsorbed onto C18 sorbent from a large volume of coconut juice samples, the eluate containing the desired compounds was used as the dispersive solvent in MC-DLLME procedure, and extraction step was largely simplified. The new method provided a high enrichment factor （319-478） for several acidic phytohormones. Good linearities were obtained for the analytes, and the developed method has also good precision and recovery, which provides application potential in the analysis of phytohormones.