基于离子液体、壳聚糖、纳米银和血红蛋白修饰电极的过氧化氢传感器的制备与研究

Preparation of Hydrogen Peroxide Sensor Based on Ionic Liquid,Chitosan Nano-Silver and Hemoglobin Modification

以离子液体1-丁基-3-甲基咪唑六氟磷酸盐(BMIMPF6)、壳聚糖(CS)、纳米银(Nano-Ag)为修饰剂,制备了Nano-Ag/CS/BMIMPF6/Au/CME修饰电极,将血红蛋白(Hb)固载在修饰电极表面,离子液体优良的导电性和Nano-Ag高的表面活性和强的吸附性,为Hb的吸附和在修饰电极表面的直接电子转移构建了一个良好的微环境,同时也提高了Hb的电催化活性,并以此制备了具有良好催化活性的H2O2生物传感器。研究了修饰电极的特性,优化了修饰电极的实验条件,详细的讨论了Hb在电极上的电子转移机理以及H2O2的电化学行为,并对实际样品中的H2O2进行了检测,获得较好结果。

The electrochemical behavior of hydrogen peroxide （Hz O2）was studied by cyclic voltammetry and Linear scanning at a gold electrode（Au/CEM） modified by hemoglobin（Hb）, chitosan（CS）, a gel containing nano-silver （Nano-Ag） and room temperature ionic liquid （RTIL） of 1-butyl-3- methylimidazolium hexafluorophosphate（BMIMPF6）. A good micro environment has been set up for Hb,and due to the,fine conductivity of ionic liquid BMIMPF6, the high surface activity and strong adsorption of nano-silver increased the adsorption and electric catalytic activity of Hb. This paper studies the characteristics of the modified electrodes, optimize the experimental conditions of modified electrodes, detailedly discuss the mechanism of Hb electron transfer on the surface of modified electrode and the electrochemical behavior of H202. The modified electrode was applied for the analysis of actual sample of HzO2 with satisfactory result.