大孔吸附树脂从藻粉中分离纯化微囊藻毒素

Separation and purification of microcystins in algae crude powder with macroporous resin

比较了XAD-2树脂和D101树脂分别从天然藻粉粗品中吸附分离微囊藻毒素(Microcystin-LR,MC-LR)的结果,讨论了用不同体积分数的乙酸、丙酮和乙醇溶液淋洗去除树脂吸附杂质的效果.实验结果表明:XAD-2树脂分离效果优于D101树脂.对于采用XAD-2树脂吸附分离得到的微囊藻毒素MC-LR,采用体积分数40%乙酸、10%丙酮和20%乙醇溶液淋洗杂质的效果较好,采用体积分数60%的甲醇水溶液洗脱微囊藻毒素MC-LR可以获得最大产品质量浓度,并且该纯化方法重现性良好.

XAD-2 resin and D101 resin were compared on adsorption separation of Microcystin-LR （MC-LR） in algae crude powder. Removal effects of impurities adsorbed on these resins with microcystins （MCs） by acetic acid, acetone and ethanol were discussed at different volume fractions. It is shown that XAD-2 resin has a better purification effect than D101 resin. When MC-LR purified by XAD-2 resin is leached by acetic acid, acetone and ethanol to remove the impurities, the optimum volume fraction is 40% for acetic acid, 10% for acetone and 20% for ethanol, respectively. When eluted with 60% methanol solution, MC-LR can reach its maximum mass fraction, and this purification method is well reproducible.