山羊CREM基因的克隆与生物信息学分析

The Clone and Bioinformatics Analysis of CREM Gene in Goat(Capra hircus)

[摘 要]研究旨在克隆山羊睾丸CREM基因cDNA全序列并进行生物信息学分析.通过提取山羊睾丸总RNA,利用qPCR和RACE技术,扩增山羊PHGPx基因cDNA序列.结果表明,山羊睾丸CREM基因cDNA全长序列为1 183 bp（登录号：HM 802260）,包含有960 bp的开放阅读框,编码319个氨基酸；山羊CREM蛋白二级结构功能区域属于bZIP转录因子家族,在319个氨基酸的序列中,73到115之间有一个“pKID”区域,259～316之间含有一个典型的“BRLZ”结构.构建蛋白质氨基酸分子进化树,结果显示山羊与牛亲缘关系最近.该研究结果将为CREM基因表达分子调控研究提供一定的理论依据.

This study aimed to clone the full length cDNA of the goat CREM gene.Total RNA was extracted from goat testis and the full length cDNA was obtained by the reverse transcription PCR and RACE method.The results showed that the full length of CREM cDNA was 1183 bp and with a 960 bp opening reading frame （ORF）,which encoding 319 amino acids.The bioinformatics predictions showed that the CREM gene belongs to the bZIP transcription factor family,and signature motifs pKID 73-115 and BRLZ 259-316 were also found.The phylogenic tree based on CREM amino acid sequence among multiple species detected the closest relationship between goat and cattle.These results would be the theoretical foundation for the further study on the genetic control of CREM gene expression.