北京及周边地区7个地理种群的亚洲小车蝗mtDNA COI基因序列分析

Sequence analysis of mtDNA COI gene of 7 geographical populations of Oedaleus asiaticus in Beijing and surrounding regions

以线粒体DNA COI基因中一段645bp的特异片段作为目的片段,对来自内蒙古自治区的锡林郭勒盟、赤峰市、乌兰察布市,河北省的张家口市、承德市和北京的密云县在内共7个地理种群的139个亚洲小车蝗样本进行了DNA序列分析。结果表明:在目的片段序列中共检测到12个多态性位点,占碱基总数的1.86%。7个种群中内蒙古赤峰广兴源种群的遗传变异程度相对较高,其核苷酸多样性值为0.000 84,河北张家口种群、河北承德种群和北京密云种群均未发生遗传变异。在139条序列中共鉴定出13种单倍型,各单倍型之间未形成与其地理种群空间位置相对应的聚类单元,并且在单倍型网络进化图中也未发现各单倍型之间存在明显的进化关系。

The 645 bp fragment of mtDNA COI gene from139 individuals of 7 geographical populations in Miyun County,Beijing and surrounding regions（including Xilingol League,Chifeng and Wulanchabu of Inner Mongolia,Zhangjiakou and Chengde of Heibei Province）were sequenced and analyzed.The results indicated that 12 genetic polymorphisms were found in the target fragments,about 1.86% of sequenced nucleotide.Variation degree of guangxingyuan population in Chifeng,Inner Mongolia was the highest among all the 7 populations.Genetic variation was not found in the populations from Zhangjiakou,Chengde and Miyun.Cluster units corresponding to geographic location were not found among 13 haplotypes identified in139 sequences,and there was no obvious evolutionary relationship among these haplotypes in the haplotype networks.