云南红皮梨bHLH转录因子的生物信息学分析

Bioinformatics Analysis of bHLH Transcription Factor in Yunnan Red Skin Pear(Pyrus pyrifolia Naki)

具有碱性-螺旋-转角-螺旋结构的一大类基因被称为bHLH家族基因,其成员主要有c-MYC、MADL、MAX、MIXL和MNT基因;bHLH转录因子在细胞的增殖、分化和凋亡方面起着重要作用;对植物的生长发育、营养吸收、生物合成和信号转导等方面同样有着重要作用。在果肉、叶片和外果皮中,CsMYC2的表达与CHS、UFGT及ANS表达相关联,因此CsMYC2参与花色苷的生物合成调控,但是该基因在云南红皮梨花色苷合成调控中起着什么样的作用尚不清楚。利用生物信息学方法对云南红皮梨(Red Pyrus pyrifolia)bHLH基因进行分析,对不同植物间bHLH基因氨基酸序列的同源性比对,并且对该基因编码的蛋白质的理化性质、结构和功能等进行预测和分析。结果表明:该蛋白属于亲水性蛋白,其二级结构中的主要结构元件是α-螺旋和无规则卷曲,并散布于整个蛋白。这一结果为研究红皮梨bHLH基因的结构、功能以及红皮梨的着色机制奠定了一定的基础。

The bHLH a basic-helix-turn-helix structure and its bHLH family members incloud c-MYC, MA DL and MAX, MIXL and MNT. bHLH transcription factors play an important role in cell proliferation, differentiation and apoptosis. It also has a vital role on plant growth and development, nutrient absorption and biosynthesis, signal transduction, etc. In the flesh, leaves and exocarp, CsMYC2 expression was associated with CHS, UFGT and ANS expression. Therefore, CsMYC2 involved in anthocyanin biosynthesis and regulation. It is not clear what the gene plays a role of anthocyanin synthesis regulation in Yunnan red-skin pear. This research investigated the bHLH gene of Yunnan red-skin pears by using bioinformatic method and software. The homology of amino acid sequences of the bHLH among different plants was compared and the physical and chemical properties, structure and function of the gene encoding protein were predicted and analyzed. The result showed that the protein was hydrophilic without signal peptide with its second structure of the protein mainly containing oL-helix and random coil, which spread randomly. The findings could be used to investigate the structure and function of the gene and to provide founda- tions for controlling effect of the bHLH gene in the coloration process of red-skin pears in further study.