不同动物油脂对大鼠血糖血脂及血液相关因子的影响研究

Effects of Different Animal Fatty Oils on Plasma Glucose,Lipids and Related Blood Factors in Rats

研究羊油、猪油、牛油、马油4种油脂对大鼠血糖、血脂及血液相关因子的影响研究。130只雄性健康SD大鼠随机分为对照组、羊油脂组、猪油脂组、牛油脂组和马油脂组共5组。各油脂组再分为高(2mL/100g体重)、中(1mL/100g体重)、低(0.5mL/100g体重)剂量3个灌胃组,对照组灌胃纯净水(1mL/100g体重)。灌胃56d后,测定各组动物体重、内脏脂肪重量、血糖(GLU)、血脂4项〔高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、胆固醇(TC)、甘油三酯(TG)〕的变化以及脂代谢相关血液因子〔血浆胰岛素(INS)、瘦素(LEP)、一氧化氮(NO)、超氧化物歧化酶(SOD)和丙二醛(MDA)〕的变化。结果表明,与对照相比,4种油脂对大鼠的体重、LEP、NO影响不明显(P>0.05);4种动物油脂的高剂量组以及猪油脂和马油脂的中剂量组均能显著增加内脏脂肪的重量(P<0.05);羊油脂中剂量组、马油脂高剂量和中剂量组的GLU值均极显著升高(P<0.01);羊油脂高剂量组HDL-C水平显著升高(P<0.05),猪油脂高剂量组HDL-C水平极显著升高(P<0.01);牛油脂高剂量组LDL-C水平显著升高(P<0.05);牛油脂和马油脂的各剂量组TC水平均极显著升高(P<0.01);仅羊油脂高剂量组TG水平显著升高(P<0.05);马油脂的高、中剂量组的血浆INS水平极显著降低(P<0.01);羊油脂和猪油脂的各剂量组MDA水平均极显著升高(P<0.01);牛油脂和马油脂的各剂量组SOD水平均极显著升高(P<0.01);4种油脂对大鼠的体重、血浆LEP、血浆NO没有明显影响;灌服猪油脂对大鼠GLU水平影响不明显;灌服高剂量羊油脂可明显升高大鼠血液HDL-C和TG水平;猪油脂主要表现为升高HDL-C水平;而牛油脂和马油脂各剂量组则可极显著升高血浆总胆固醇。羊油脂和猪油脂均能升高大鼠的MDA水平;牛油脂和马油脂均能升高大鼠的SOD水平,推测牛油脂和马油脂具有抗氧化的作用。

To study the effect of four kinds of animal fatty oils on the plasma glucose and lipids and relat- ed factors in the blood of rats,169 male SD rats were randomly divided into 5 groups,namely,the control group, the ovine fatty oil, swine fatty oil, bovine fatty oil and equine fatty oil fed groups, each of which was divided in to three sub-groups fed with different doses oI fatty oils,namely,high （2mL/100 g.bw） ,medium （lmL/100 g. bw） and low dose （0. S mL/100 g. bw）, and the control group was fed with purified water （ 1 mL / 1 0 0 g. bw ） intragastrally. After 5 6 - day feeding experiment , the body weight , visceral fat weight ,plasma glucose （GLU）,four plasma lipids i. e. ,high density lipoprotein （HDL-C） and low density lipopro rein （LDL-C） ,cholesterol （TC） ,triglyceride （TG） and the factors in the blood associated with lipid metab- olism i.e., insulin （INS）, leptin （LEP）, nitricoxide （NO）, superoxidedismutase （SOD）, malondialdehyde （MDA） were measured.There were no significant differences in the animal＇s body weight and LEP,NO between different animal fatty oils fed group and the control group,however the visceral fat weight in rats fed with high dose of different fatty oils as well as in rats fed with medium dose of swine and equine fatty oils was increased significantly （P 〈0.05）.The plasma glucose content was extremely increased in rats fed with medium dose of ovine fatty oil,high and medium dose of equine fatty oils were much more than that in the control （P 〈0.01） ,and significantly increased in the rat fed with low dose of ovine fatty oil and high dose of bovine oil （P 〈0.05）.The plasma HDL-C content in the rat fed with high dose of ovine fatty oil was significantly increased （P 〈0.05）,and extremely increased in the rat fed with high dose of swine fatty oil （P 〈0.01） ,whereas only high dose of bovine fatty oil feeding stimulated rat＇s LDIzC increasing signifi- cantly （P 〈0.05）.The plasma TC content in rats fed with either bovine or equine fatty oils was extremely increased （P 〈0.01） ,and only high dose of ovine fatty oil feeding could stimulate rat＇s TG increasing sig- nificantly （P 〈0.05）.The insulin was extremely decreased in the rat fed with high and medium dose of e quine fatty oil （P 〈0.01）.The melondialdehyde was extremely decreased in the rat fed with ovine and swine fatty oils （P 〈0.01）.The superoxide dismutase was extremely decreased in the rat fed with bovine and equine fatty oils （P 〈0.01）.Four animal fatty oils had no effect on the body weight,LEP and NO.Dif ferent animal fatty oils had different impact on plasma glucose and plasma lipids.-ovine fatty oil raised plas- ma HDL-C and TG level, swine fatty oil raised plasma HDL-C level,however, bovine and equine fatty oils increased plasma total cholesterol level extremely.Different animal fatty oils had no impact on LEP,where- as ovine and swine fatty oils elevated the plasma melondiatdehyde,and bovine and equine fatty oils could significantly increase SOD level suggesting bovine and equine fatty oils had antioxidant effect.