光谱法研究辅酶Q10与环糊精衍生物的相互作用

Study on inclusion interaction of coenzyme Q10 and cyclodextrins using spectroscopic methods

采用共沉淀法和共蒸发法制备辅酶Q10分别与β-CD,HP-β-CD及DM-β-CD的包合物,并分别用红外光谱法,紫外分光光度法和核磁共振法对包合物进行表征.结果表明:辅酶Q10与环糊精通过非键作用形成1∶1包结物,其结合常数大小次序为β-CD>HP-β-CD>DM-β-CD,这表明β-CD包结辅酶Q10的能力大于HP-β-CD及DM-β-CD.包合反应过程中,ΔG0<0,ΔH0>0,ΔS0>0,说明辅酶Q10与环糊精的包合可以自发进行并且是个吸热反应.而且,辅酶Q10与这三种环糊精(β-CD,DM-β-CD和HP-β-CD)相互作用形成包结物时,均是辅酶Q10的苯环插入环糊精空穴内,且都是从环糊精的大口端进入.

In this article, CoQ10-β-CD, CoQ10-HP-β-CD and CoQ10-DM-β-CD complexes were prepared by co-precipitate and co-evaporated methods, respectively. The inclusion interactions of CoQ10 with CDs were characterized by the infrared spectra （FT-IR）, ultraviolet spectrophotometry （UV） and nuclear magnetic resonance （NMR）. The results showed that the inclusion complexes of CoQ10 with cyclodextrins were formed by the non-bond interactions. The stoichiometry for these complexes is 1 ： 1. The association constant （Ka） is in the order of β-CD〉HP-13-CD-β-DM-13-CD, indicating that β-CD had stronger inclusion ability than HP-β-CD and DM-13-CD and the cavity size of β-CD ？ was suitable for the inclusion with CoQ10. In the inclusion process of CoQ10 with cyclodextrins,△G^0〉0, △H^0〉0, △S^0〉0, indicating that the inclusion interaction of CoQ10 with cyclodextrins was an endothermic and spontaneous process. Moreover the spectroscopic results showed that the benzene ring of CoQ10 was probably inserted into the cavity of CDs and all inserted from the wide rim of CDs.