摘要
采用胶体金测试条法对根食中呕吐毒素进行快速定量检测,结果表明,呕吐毒素的检测限为118μg/kg,定量限为316μg/kg,500-5 000 μg/kg质量浓度范围内,相关系数:为0. 9987.采用有证实物参考物质进行比较,正确度为90. 3%一116.6%,变异系数为4. 7%一19.6%.采用配对t-检验,与高效液相色谱法的测定结果相比较,二者无显著差异.
The colloidal gold strips method was used for the rapid quantitative detection of vomitoxin in grain. The results showed that the detection limit of vomitoxin was 118μg/kg. The quantitation limit was 316 μg/kg and the correlation coefficient (r) was 0. 998 7 in the range of 500 -5 000 μg/kg. Compared with the certified reference material, the accuracy was in the range of 90. 3% -116.6% and the coefficient of variation was ranged from 4.7% to 19.6%. There were no significant differences between the colloidal gold strips and the high-performance liquid chromatography using the paired t-test.
出处
《食品科学技术学报》
CAS
2013年第4期55-58,共4页
Journal of Food Science and Technology
基金
北京市委
市政府重点工作及区县政府应急项目(Z111100056811003)
关键词
胶体金
呕吐毒素
粮食
快速定量
colloidal gold
vomitoxin
grains
rapid quantitative