污水中解有机磷细菌的筛选及解磷能力测定

Screening and Determination Degradation Ability of Organic Phosphate-solubilizing Bacteria in Sewage

采集生活污水,从中分离出具有解有机磷能力的菌株,纯化得到14株解有机磷细菌。通过平板法测定溶磷圈后,筛选出4株解磷能力较强的菌株进一步做解磷能力的定量测定。结果发现,OPB8、OPB17、OPB15、OPB19菌株达到解磷峰值时,解磷率分别为107.9%、135.1%、31.8%和249.2%,随着培养时间的延长,培养液中的水溶性磷含量均有下降,其中OPB15、OPB19菌株的培养液中的水溶性磷含量分别降低了72.2%和95.7%。

Organophosphorus solid medium was adopted to isolate strains that were capable of solving organophosphorus from sewage. 14 strains of organic phosphate-solubilizing bacteria were obtained by purification. The dissolved phosphorus transparency circle could be determined through plate method, and 4 phosphate-solubilizing bacteria were selected to determine quantitative degradation ability by plate method. Phosphate-solubilizing peaking rate of OPB8, OPB17, OPB15, OPB19 strain were 107.9%, 135.1%, 31.8% and 249.2%. Then, the water-soluble phosphorus concentration dropped in the nutrient solution, of which OPB 15, OPB19 strain of wa- ter-soluble phosphorus concentration in the nutrient solution dropped by 72.2% and 72.2% respectively.