凝溶胶蛋白在C57BL/6小鼠急性放射肺损伤中的表达

Gelsolin expression of acute radiation lung injury in C57BL/6 mice

为研究小鼠辐射诱导肺损伤后凝溶胶蛋白(Gelsolin,GSN)在血浆和肺组织中的表达,分别采用4 Gy和20Gy剂量的X射线对C57BL/6小鼠进行全身及胸部单次照射。且在照射后取不同时间点ELISA Kit检测小鼠血浆型Gelsolin(Plasma GSN,pGSN)蛋白含量和Western blot法检测小鼠肺组织胞浆型Gelsolin(Cytoplasmic GSN,cGSN)蛋白含量。同时,检测20 Gy X射线胸部照射后不同时间点小鼠右肺肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)中的细胞总数和总蛋白的含量。结果表明,全身照射组小鼠在照射后24 h时,pGSN水平降低,而胸部照射组pGSN水平在24-48 h持续降低,下降速度慢于全身照射组,之后两组pGSN均逐渐上升。在照射后24 h时小鼠肺组织cGSN含量低于正常水平,照射后48 h时则高于正常水平,48-96 h持续下降。但在照射后96 h时全身照射组cGSN含量恢复到正常,胸部照射组仍高于正常水平。小鼠胸部照射后在24 h时,右肺BALF中细胞总数和总蛋白浓度显著高于正常水平,约达正常值的16倍,24-96 h持续下降,与肺组织cGSN含量变化存在一定程度的负相关。推测GSN可能促进急性放射肺损伤的修复。

To investigate the changes in plasma and cytoplasmic gelsolin during radiation-induced acute lung injury, C57BL/6 mice were randomly divided into two groups for irradiation： the total body irradiation with 4 Gy and the thoracic irradiation with 20 Gy. The plasma gelsolin （pGSN） levels were detected by ELISA Kit and the cytoplasmic gelsolin （cGSN） of the lung tissue were detected by western Blot. The total cell number and the protein concentration of bronchoalveolar lavage fluid （BALF） in the right lung of thoracic irradiated mice with 20 Gy were detected. The results show that the pGSN levels in total body irradiated mice with 4 Gy decrease significantly at 24 h post-radiation. In contrast, the pGSN levels in thoracic irradiated mice with 20 Gy decline more slowly but decrease significantly at 48 h post-radiation. After that, the pGSN levels in both groups begin to recover slowly. The cGSN levels in lung tissue of both groups decrease at 24 h post-radiation, but increase significantly at 48 h post-radiation, then gradually recover. But the changes in cGSN levels of thoracic irradiated mice are more significant. The total cell number and the protein concentration of BALF in the right lung of mice treated by the thoracic irradiation with 20 Gy increase significantly at 24 h post-radiation, approximately 16 times of the control＇s, then decrease and gradually become stabilized. There is a negative correlation with the cGSN levels in lung tissue of thoracic irradiated mice with 20 Gy. pGSN and cGSN may play an important role in the process of repairing acute lung injury.