脾肺气虚证HIV/AIDS患者CD_8^+CD_(38)^+T淋巴细胞及自然杀伤细胞变化的分析

Research on CD_8^+CD_(38)^+T Lymphocytes and Natural Killer Cell in Qi Deficiency of Spleen and Lung of HIV/AIDS Patients

目的:探索HIV/AIDS脾肺气虚证患者T淋巴细胞活化及自然杀伤细胞数量变化。方法:对河南省某地区的HIV/AIDS患者进行调查,筛选出脾肺气虚证患者26例,并在该地区筛选出20例健康人作为对照。采集患者和健康者的抗凝血液。应用流式细胞术检测外周血中CD_8^+T淋巴细胞绝对数量以及CD_8^+CD_(38)^+T和CD_(56)^+CD_(16)^+CD_3^-细胞的绝对数量和百分率。结果:和健康组比较,脾肺气虚组CD_8^+T和CD_8^+CD_(38)^+T细胞数量和百分率均显著升高(P<0.05-0.01);CD_(56)^+CD_(16)^+CD_3^-细胞数量和百分率均显著降低(P<0.01)。结论:脾肺气虚证患者CD_8^+T淋巴细胞显著活化。NK细胞数量明显下降,提示:脾肺气虚证患者的天然免疫功能受到一定的损害。

Objective ： To assay CDs CD38＋ T lymphocyte and natural killer cell of HIV/AIDS patients with Qi deficiency of spleen and lung. Method： Twenty - six HIV/AIDS patients with Qi - deficiency of spleen and lung were selected to study in Henan province, with 20 samples of healthy donors in the area. Anti - coagulated blood was collected in all the samples to assay CD8＋ CD38＋ T lymphocyte and natural killer cell in peripheral blood. Results： Compared with healthy group, CDs CD38＋T lymphocyte count and CDsCD38＋ T/CD8＋ T ratio were significantly higher in the Qi deficiency of spleen and lung patients （P 〈0.05-0.01 ）, however, NK cell count and percent in lymphocyte were significantly lower （P 〈 0.05 -0.01）. Conclusion： CD8＋T lymphocyte is activated and the function of innate immune is low in HIV/AIDS pa- tients with Qi deficiency of spleen and lung.